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At the beginning of day three, the spacecraft was about away from the Earth.
The velocity of the craft steadily decreased, as Apollo 16 had not yet reached the lunar sphere of gravitational influence.
The early part of day three was largely housekeeping, spacecraft maintenance and exchanging status reports with mission control in Houston, Texas.
The crew performed the Apollo light flash experiment, or ALFMED, to investigate " light flashes " that were seen by the astronauts when the spacecraft was dark, regardless of whether or not their eyes were open, on Apollo lunar flights.
This was thought to be caused by the penetration of the eye by cosmic ray particles.
During the second half of the day, John Young and Charlie Duke again entered the Lunar Module to power it up and check its systems, as well as perform housekeeping tasks in preparation for lunar landing.
The systems were found to be functioning as expected.
Following this, the crew donned their spacesuits and rehearsed procedures that would be used on landing day.
Just before the end of flight day three at 59 hours, 19 minutes, 45 seconds after liftoff, while from the Earth and from the Moon, the spacecraft's velocity began increasing as it accelerated towards the Moon after entering the lunar sphere of influence.

1.868 seconds.