Page "ELISA" Paragraph 1

The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.

Attempting to detect ( and quantify ) the presence of the antigen in the sample proceeds as follows: Antigens from the sample are attached to a surface.

Then, a further specific antibody is applied over the surface so it can bind to the antigen.

This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added.

The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.