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Recent progress on automated population identification using computational methods has offered an alternative to traditional gating strategies.
Automated identification systems could potentially help findings of rare and hidden populations.
Representative automated methods include FLOCK in Immunology Database and Analysis Portal ( ImmPort ), FLAME in GenePattern and flowClust, in Bioconductor.
Collaborative efforts have resulted in an open project called FlowCAP ( Flow Cytometry: Critical Assessment of Population Identification Methods ,) to provide an objective way to compare and evaluate the flow cytometry data clustering methods, and also to establish guidance about appropriate use and application of these methods.

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