Page "Fluorescence" Paragraph 53

Fluorescence in several wavelengths can be detected by an array detector, to detect compounds from HPLC flow.

Also, TLC plates can be visualized if the compounds or a coloring reagent is fluorescent.

Fluorescence is most effective when there is a larger ratio of atoms at lower energy levels in a Boltzmann distribution.

There is, then, a higher probability of excitement and release of photons by lower-energy atoms, making analysis more efficient.