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On his first entrance into professional life Anstie was occupied in administering chloroform for the operations of Sir William Fergusson ; but he soon went into practice as a physician, and became very fully occupied in hospital work and in journalism, being for some years a member of the editorial staff of the The Lancet ; while in the last few years of his life he was beginning to get a good consulting practice.
Dr. Anstie's life was cut short by an illness contracted in the course of a sanitary inspection.
Some strange cases of fatal disease having occurred in the schools of the Patriotic Fund at Wandsworth, Anstie was called in to make an inspection of the buildings and investigate the causes of the epidemic.
In making a post-mortem examination he received a slight wound, from the effects of which he died on 12 September 1874.

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