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Golgi ' staining was famously used by Spanish neuroanatomist Santiago Ramón y Cajal ( 1852 – 1934 ) to discover a number of novel facts about the organization of the nervous system, inspiring the birth of the neuron doctrine.
Ultimately, Ramon y Cajal improved the technique by using a method he termed " double impregnation.
" Ramon y Cajal's staining technique, still in use, is called Cajal's Stain.

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