Page "Intracytoplasmic sperm injection" Paragraph 5
from
Wikipedia
The procedure is done under a microscope using multiple micromanipulation devices ( micromanipulator, microinjectors and micropipettes ).
A holding pipette stabilizes the mature oocyte with gentle suction applied by a microinjector.
From the opposite side a thin, hollow glass micropipette is used to collect a single sperm, having immobilised it by cutting its tail with the point of the micropipette.
The oocyte is pierced through the oolemma and into the inner part of the oocyte ( cytoplasm ).
The sperm is then released into the oocyte.
The pictured oocyte has an extruded polar body at about 12 o ' clock indicating its maturity.
After the procedure, the oocyte will be placed into cell culture and checked on the following day for signs of fertilization.
Page 1 of 1.
2.097 seconds.