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On 20 April 1564, it became the port of departure for the French expedition of René Goulaine de Laudonnière to the New World where he created the first French colony at Fort Caroline near present-day Jacksonville, Florida.
Famed artist Jacques le Moyne de Morgues joined Laudonnière on this colonizing effort and created the first known artistic depictions by a European of Native Americans in the New World, specifically the Timucua tribes in the modern-day areas of northeast Florida and southeast Georgia.

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