Page "Neurotransmitter" Paragraph 2
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The chemical identity of neurotransmitters is often difficult to determine experimentally.
For example, it is easy using an electron microscope to recognize vesicles on the presynaptic side of a synapse, but it may not be easy to determine directly what chemical is packed into them.
The difficulties led to many historical controversies over whether a given chemical was or was not clearly established as a transmitter.
In an effort to give some structure to the arguments, neurochemists worked out a set of experimentally tractable rules.
According to the prevailing beliefs of the 1960s, a chemical can be classified as a neurotransmitter if it meets the following conditions:
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