Page "Protein" Paragraph 16

Short proteins can also be synthesized chemically by a family of methods known as peptide synthesis, which rely on organic synthesis techniques such as chemical ligation to produce peptides in high yield.

Chemical synthesis allows for the introduction of non-natural amino acids into polypeptide chains, such as attachment of fluorescent probes to amino acid side chains.

These methods are useful in laboratory biochemistry and cell biology, though generally not for commercial applications.

Chemical synthesis is inefficient for polypeptides longer than about 300 amino acids, and the synthesized proteins may not readily assume their native tertiary structure.

Most chemical synthesis methods proceed from C-terminus to N-terminus, opposite the biological reaction.