Page "Protein" Paragraph 50

Other methods for elucidating the cellular location of proteins requires the use of known compartmental markers for regions such as the ER, the Golgi, lysosomes / vacuoles, mitochondria, chloroplasts, plasma membrane, etc.

With the use of fluorescently tagged versions of these markers or of antibodies to known markers, it becomes much simpler to identify the localization of a protein of interest.

For example, indirect immunofluorescence will allow for fluorescence colocalization and demonstration of location.

Fluorescent dyes are used to label cellular compartments for a similar purpose.