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Thomas Bartholin came from a family that has become famous for its pioneering scientists, twelve of whom became professors at the University of Copenhagen.

Three generations of the Bartholin family made significant contributions to anatomical science and medicine in the 17th and 18th centuries: Thomas Bartholin's father, Caspar Bartholin the Elder ( 1585-1629 ), his brother Rasmus Bartholin ( 1625-1698 ), and his son Caspar Bartholin the Younger ( 1655 – 1738 ).