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Two metabolites ( 1, and 2 ) ) of p-aminobenzoic acid ( PABA ) which act as cofactors for the hydroxylation of aniline by acid-fast bacteria are biosynthesized from Aj.

The 7 carbons of PABA are incorporated directly into metabolite 2 ( ( as shown with both ring-labeled and carboxy-labeled Af ).

Thirty-five of the 36 carbon atoms arise from Aj.

All 28 carbons of metabolite 1 ( ( a product of mild acid hydrolysis of 2 ) arise from Aj.

Metabolite 1, isolated from the medium, however, showed a lower specific activity, which indicates endogenous synthesis of this metabolite.