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A variety of techniques have been directed toward the isolation and study of blood group antibodies.
These include low-temperature ethanol ( Cohn ) fractionation, electrophoresis, ultracentrifugation and column chromatography on ion exchange celluloses.
Modifications of the last technique have been applied by several groups of investigators.
Abelson and Rawson, using a stepwise elution scheme, fractionated whole sera containing ABO and Rh antibodies on diethylaminoethyl DEAE cellulose and carboxymethyl cellulose.
Speer and coworkers, in a similar study of blood group antibodies of whole sera, used a series of gradients for elution from Aj.
Fahey and Morrison used a single, continuous gradient at constant pH for the fractionation of anti-A and anti-B agglutinins from preisolated **yg-globulin samples.
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