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Serum samples were obtained from normal group A, group B and group O donors.
Three of the anti-Rh sera used were taken from recently sensitized individuals.
One contained complete antibody and had a titer of 1: 512 in saline.
The second contained incomplete antibody and showed titers of 1: 256 in albumin and 1: 2048 by the indirect Coombs test.
The third, containing the mixed type of complete and incomplete antibodies, had titers of 1: 256 in saline, 1: 512 in albumin and 1: 1024 by the indirect Coombs test.
In addition one serum was obtained from a donor ( R. E. ) who had been sensitized 6 years previously.
This serum exhibited titers of 1: 16 in albumin and 1: 256 by the indirect Coombs test.
These antibody titers were determined by reaction with homozygous Af red cells.
Serological technique.
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