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from Brown Corpus
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When paper electrophoresis was to be used for preparation, eight strips of a whole serum sample or a chromatographic fraction concentrated by negative pressure dialysis were run/chamber under the conditions described above.
At the end of the run, the strips in the third and sixth positions in each chamber were dried, stained for 1 hr, washed and dried, while the other strips were maintained in a horizontal position at 1-degree-C.
The unstained strips were then marked, using the stained ones as a guide, and cut transversely so as to separate the various protein bands.
The strip sections containing a given protein were pooled, eluted with 0.5 ml of isotonic saline, and the eluates were tested for antibody activity.
Ultracentrifugation.

1.987 seconds.