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Not all these regions exhibited equal agglutinating activity, as evidenced by titer and the extent of the active areas.

In all cases, most of the activity lay in the region of high anionic binding capacity.

This was particularly noticeable in group A and group B sera, in which cases activity in Regions 1 and 2 was usually not detectable without prior concentration and occasionally could not be detected at all.

There appeared to be no difference in the distribution of anti-A and anti-B activity in group O serum, though in two group O donors ( J. F. and E. M. ) only one type of agglutinin was found in the regions of low anionic binding capacity ( Figs. 1 and 2 ).