The first step in immunoperoxidase staining is the binding of the specific ( primary ) antibody to the cell or tissue sample.

The detection of the primary antibody can be then accomplished directly ( example 1 ) or indirectly ( examples 2 & 3 ).

The primary antibody can be directly tagged with the peroxidase enzyme which is then used to catalyse a chemical reaction to generate a coloured product.

The primary antibody can be tagged with a small molecule that can be recognised by a peroxidase-conjugated binding molecule with high affinity.

The most common example of this is a biotin linked primary antibody that binds to an enzyme-bound streptavidin.

An untagged primary antibody is detected using a general secondary antibody that recognises all antibodies originating from same animal species as the primary.

Alternatively, the primary antibody can be detected using fluorescent label ( immunofluorescence ), or be attached to colloidal gold particles for electron microscopy.

The extramitochondrial localization of cytochrome c was shown to be specific as it was completed abolished upon adsorption of the primary antibody with the purified cytochrome c. The presence of cytochrome-c outside of mitochondria at specific location under normal physiological conditions raises important questions concerning its cellular function and translocation mechanism.

This primary antibody could also be in the serum of a donor to be tested for reactivity towards the antigen.

* A secondary antibody is added, which will bind the primary antibody.

The color change shows the secondary antibody has bound to primary antibody, which strongly implies the donor has had an immune reaction to the test antigen.

* The higher the concentration of the primary antibody present in the serum, the stronger the color change.

Within common-sense limitations, the enzyme can go on producing color indefinitely, but the more primary antibody is present in the donor serum, the more secondary antibody + enzyme will bind, and the faster the color will develop.

The image to the right includes the use of a secondary antibody conjugated to an enzyme, though, in the technical sense, this is not necessary if the primary antibody is conjugated to an enzyme.

# The secondary antibody, specific to the primary antibody, is added.

The labeled antigen competes for primary antibody binding sites with the sample antigen ( unlabeled ).

Too many people think that the primary purpose of a higher education is to help you make a living ; ;

This is the primary function of the imagination operating in the absence of the original experiential stimulus by which the images were first appropriated.

Neither is primary experience understood according to the attitude of modern empiricism in which nothing is thought to be received other than signals of sensory qualities producing their responses in the appropriate sense organs.

The principal defender of this view of primary experience as `` causal efficacy '' is Alfred North Whitehead.

Speaking as a non-Jew I believe that its primary contribution is in the realm of future policy.

The natural and primary aesthetic attitude is to enjoy contemporary art, to despise and dislike the art of the recent past, and wholly to ignore everything else ''.

Here we may observe that at least one modern philosophy of history is built on the assumption that ideas are the primary objectives of the historian's research.

But competent observers believe he is making progress, particularly toward what Sen. Jackson lists as the primary need -- `` a clearer understanding of where our vital national interests lie and what we must do to promote them ''.

Its primary advantage is that it is a moral choice ; ;

A primary function is the operation of a Government Bid Center, which receives bids daily from the Federal Government's principal purchasing agencies.

If the Department of State is to take primary responsibility for foreign policy in Washington, it follows that the ambassador is expected to take charge overseas.

For this reason, the more uncertain skywave service was denominated `` secondary '' in our rules, as compared to the steadier, more reliable groundwave `` primary service '', and, for both skywave service and skywave interference, signal strength is expressed in terms of percentage of time a particular signal-intensity level is exceeded -- 50 percent of the time for skywave service, 10 percent of the time for skywave interference.

As mentioned, the primary allocation objective to be followed in the allocation of stations on clear channels is the provision of widespread service, free from destructive interference.

The result is a color picture of the specimen where the primary colors correspond to the three different ultraviolet wavelengths.

A primary ingredient in these fields is imagination, and Tri-State Pipeline Corporation deserves a very good mark.

The theorem which we prove is more general than what we have described, since it works with the primary decomposition of the minimal polynomial, whether or not the primes which enter are all of first degree.

If Af are the projections associated with the primary decomposition of T, then each Af is a polynomial in T, and accordingly if a linear operator U commutes with T then U commutes with each of the Af, i.e., each subspace Af is invariant under U.

In the primary decomposition theorem, it is not necessary that the vector space V be finite dimensional, nor is it necessary for parts ( A ) and ( B ) that P be the minimal polynomial for T.

Though there is obviously great need for continued experimentation with various types of short-term intervention to further efforts in developing an operational definition of prevention at the secondary -- or perhaps, in some instances, primary -- level, the place of short-term intervention has already been documented by a number of investigators in a wide variety of settings.

Another release of the 6x86, the 6x86MX, added MMX compatibility, introduced the EMMI instruction set, and quadrupled the primary cache size to 64 KB.

McVeigh added more explosives to the driver's side of the cargo bay, which he could ignite ( killing himself in the process ) at close range with his Glock 21 pistol in case the primary fuses failed.

Every secondary color is the complement of one primary color ; when a primary and its complementary secondary color are added together, the result is white: cyan complements red, magenta complements green, and yellow complements blue.

The primary cast for First Contact is from the Star Trek: The Next Generation television series, to which the film's producers added Alice Krige, Neal McDonough, James Cromwell and Alfre Woodard.

It is similar to other systems engineering activities in that its primary motivation is to support the delivery of engineering solutions that satisfy pre-defined functional and user requirements, but with the added dimension of preventing misuse and malicious behavior.

The primary method for singers to accomplish this is through the use of the Singer's Formant, which has been shown to be a resonance added to the normal resonances of the vocal tract above the frequency range of most instruments and so enables the singer's voice to carry better over musical accompaniment.

The AS / 400 added source compatibility with the System / 36 combining the two primary computers manufactured by the IBM Rochester plant.

In order to reproduce it, a small amount of cyan printer's ink must be added to printer's magenta to make fuchsia, and therefore fuchsia is not a primary color of pigment — it is the color of printer's magenta that is one of the primary colors of pigment ( along with cyan and yellow ).

Flavorful ingredients like fruit purées, nuts, dried or candied fruit, or extracts are often added, and numerous substitutions for the primary ingredients are possible.

This system added the San Gabriel Fault as a primary focus of movement between 10 – 5 Ma.

For any given light stimulus, regardless of the form of its spectral emittance curve, there always exists a unique mixture of three " primary " lights that when added together, or added to the stimulus, will exactly match it.

Only the discontinued HD DVD system directly supported E-AC-3, though Blu-ray Disc offers E-AC-3 as an option to graft added channels onto an otherwise 5. 1 AC-3 stream, as well as for delivery of secondary audio content ( e. g. director's commentary ) that is intended to be mixed with the primary audio soundtrack in the Blu-ray Disc player.

Note that for conversion from heights above the surface to distances between an orbit and its primary, the radius of the central body has to be added, and conversely.

In the context of ceramics, for example, an appliqué is a separate piece of clay added to the primary work, generally for the purpose of decoration.

Previous experiences had demonstrated that till ( boulder clay ), rather than just sand or clay, was the best primary material for a structure like the Afsluitdijk, with the added benefit that till was in plentiful supply in the area ; it could be retrieved in large quantities by simply dredging it from the bottom of the Zuiderzee.

This is because of the difficulties in centering and focusing sometimes very dim objects in the narrow field of view, contending with magnified vibration and tracking errors, and the added expense of equipment ( such as sufficiently sturdy telescope mounts, camera mounts, camera couplers, off axis guiders, guide scopes, illuminated cross-hairs, or auto-guiders mounted on primary telescope or the guide-scope.

When he learned that a teacher at his old primary school was having his students analyse Beatles ' lyrics, he added a verse of nonsense words.

In their tradition, the canons added the commitment of religious vows to their primary vocation of pastoral care.

As Microsoft develops a software product new functions or features are added, major new features may be indicated with a new primary version number, minor functions or features will increment the secondary version number.