Cytotoxicity can also be measured by the sulforhodamine B ( SRB ) assay, WST assay and clonogenic assay.

* Determining the basic effects of a control treatment ( such as a dose-survival curve in clonogenic assay )

These properties can be illustrated with relative ease in vitro, using methods such as clonogenic assays, where the progeny of a single cell is characterized.

Properties of stem cells can be illustrated in vitro, using methods such as clonogenic assays, in which single cells are assessed for their ability to differentiate and self-renew.

The word " clonogenic " refers to the fact that these cells are clones of one another.

This assay is used to disrupt gene function by creating an intragenic gene fusion.

The Ames test is a biological assay to assess the mutagenic potential of chemical compounds.

Another setup commonly used for measuring chemotaxis and chemokinesis utilizes the under-agarose cell migration assay, whereby a layer of agarose gel is placed between a cell population and a chemoattractant.

A novel assay that mimics dry bacterial exposure to touch surfaces was developed because this test method is thought to more closely replicate real world touch surface exposure conditions.

In fact, the enzyme-linked immunosorbent assay ( ELISA ), which uses antibodies, is currently one of the most sensitive tests modern medicine uses to detect various biomolecules.

A subsequent review of these tests by the Federal Insecticide, Fungicide, and Rodenticide Act Scientific Advisory Panel points out that while " the negative results decrease the probability that the Cry9C protein is the cause of allergic symptoms in the individuals examined ... in the absence of a positive control and questions regarding the sensitivity and specificity of the assay, it is not possible to assign a negative predictive value to this "

Culture remains useful in selected circumstances and is currently the only assay approved for testing non-genital specimens.

Genotyping is the process of elucidating the genotype of an individual with a biological assay.

The basic idea of microfluidic biochips is to integrate assay operations such as detection,

Screening for PKU is done with bacterial inhibition assay ( Guthrie test ), immunoassays using fluorometric or photometric detection, or amino acid measurement using tandem mass spectrometry ( MS / MS ).

#" Elevated serum prolactin assay, when measured in the appropriate clinical setting at 10 to 20 minutes after a suspected event, is a useful adjunct for the differentiation of generalized tonic-clonic or complex partial seizure from psychogenic nonepileptic seizure among adults and older children ( Level B ).

The effort expended in separating a mass F of feed of assay xf into a mass P of product assay xp and waste of mass W and assay xw is expressed in terms of the number of separative work units needed, given by the expression SWU

This easy assay, which can be seen with the naked eye, without the aid of instruments, is possible because catalase has a very high specific activity, which produces a detectable response.

Bioassay ( commonly used shorthand for biological assay ), or biological standardization is a type of scientific experiment.

This is also known as the " all or none " response assay.

This assay is applied when the sample size is too small.

Since the assay does not involve the recording of concentration response curve, the sensitivity of the preparation is not taken into consideration.

A fasting serum insulin level of greater than the upper limit of normal for the assay used ( approximately 60 pmol / L ) is considered evidence of insulin resistance.

An example that is often used in teaching laboratories is a controlled protein assay.

The assay is a colorimetric assay in which a spectrophotometer can measure the amount of protein in samples by detecting a colored complex formed by the interaction of protein molecules and molecules of an added dye.

The technique allowed detection of PrP < sup > Sc </ sup > after many fewer cycles of conversion than others have achieved, substantially reducing the possibility of artefacts, as well as speeding up the assay.

This parallel artificial membrane permeability assay PAMPA technique measures the permeability across specifically formulated lipid cocktail ( s ) found to be highly correlated with Caco-2 cultures, the gastrointestinal tract, blood – brain barrier and skin.

* Pull-down assay, a biochemical protein extraction technique

The technique allowed detection of PrP < sup > Sc </ sup > after many fewer cycles of conversion than others have achieved, substantially reducing the possibility of artefacts, as well as speeding up the assay.

The technique for protein sequence determination relied upon the purification, sequencing, and cloning of the transporter protein in question, or expression cloning strategies in which transport function was used as an assay for cDNA species coding for that transporter.

The technique allowed detection of PrP < sup > Sc </ sup > after many fewer cycles of conversion than others have achieved, substantially reducing the possibility of artefacts, as well as speeding up the assay.

The HIV proteins used in western blotting can be produced by recombinant DNA in a technique called recombinant immunoblot assay ( RIBA ).

The number of cells which secrete those particular substances can be determined using a related technique, the ELISPOT assay.

As noted above, the ELISPOT assays employ a technique very similar to the sandwich enzyme-linked immunosorbent assay ( ELISA ) technique.

A DNase footprinting assay is a DNA footprinting technique from molecular biology / biochemistry that detects DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic cleavage.

Although this technique can provide accurate results, the assay is time-consuming to set up and analyse and can only provide data on tumor cells that can grow in culture.

* Comet assay, an experimental technique for measuring DNA damage

An overlay assay is a biological technique used to find proteins that bind to a protein of interest.

Radioimmunoassay ( RIA ) is a very sensitive in vitro assay technique used to measure concentrations of antigens ( for example, hormone levels in the blood ) by use of antibodies.

The diverse features of the chemotaxis receptors and ligands allows for the possibility of selecting chemotactic responder cells with a simple chemotaxis assay.

Rapid diagnostic assay methods are available for the identification of multiple drug-resistant cases.

The most common tests for ACPAs are the anti-CCP ( cyclic citrullinated peptide ) test and the Anti-MCV assay ( antibodies against mutated citrullinated Vimentin ).

This assay combines the detection of rheumatoid factor and anti-MCV for diagnosis of rheumatoid arthritis and shows a sensitivity of 72 % and specificity of 99. 7 %.

A physician may order a metal assay for a number of reasons, such as suspicion of heavy metal poisoning, metabolic concerns, and even hepatological issues.

In patients with a clinical suspicion for Tay – Sachs disease, with any age of onset, the initial testing involves an enzyme assay to measure the activity of hexosaminidase in serum, fibroblasts or leukocytes.

Because Tay – Sachs disease was one of the first autosomal recessive genetic disorders for which there was an enzyme assay test ( prior to polymerase chain reaction testing methods ), it was intensely studied as a model for all such diseases, and researchers sought evidence of a selective process.

Organ chemiluminescence: Noninvasive assay for oxidative radical reactions.

Negative control samples would contain all of the reagents for the protein assay but no protein.

For cystic echinococcosis, imaging is the main method that is relied on for diagnosis while serology tests ( such as indirect hemogglutination, ELISA ( enzyme linked immunosorbent assay ), immunoblots or latex agglutination ) that use antigens specific for E. granulosus are used to verify the imaging results.

Currently, SUNY ESF has over 100 individual events being tested, with more than 400 slated to be in the field or in the lab for various assay tests in the next several years.

There are a number of diagnostic tests for including: HCV antibody enzyme immunoassay or ELISA, recombinant immunoblot assay, and quantitative HCV RNA polymerase chain reaction ( PCR ).

Another report using a different assay for assessing antioxidant strength placed blackberry at the top of more than 1000 antioxidant foods consumed in the United States.

Bituminous coal must meet a set of criteria for use as coking coal, determined by particular coal assay techniques.

Bituminous coal must meet a set of criteria for use as coking coal, determined by particular coal assay techniques.

During severe liver damage, xanthine oxidase is released into the blood, so a blood assay for XO is a way to determine if liver damage has happened.

Nonetheless, Eakins found a subject which referenced his native city, an earlier Philadelphia artist, and allowed for an assay on the female nude seen from behind.