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A reaction consisting of the histone octamers and a naked DNA template can be incubated together at a salt concentration of 2 M. By steadily decreasing the salt concentration, the DNA will equilibrate to a position where it is wrapped around the histone octamers, forming nucleosomes.
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reaction and consisting
The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA.
The soundtrack features music and imagined dialogue scripted by Greenaway for the 126 " wedding guests, servants, onlookers and wedding crashers " depicted in the painting, consisting of small talk and banal chatter that culminates in reaction to the miraculous transformation of water to wine, according to the Gospels the first miracle performed by Jesus.
The movement developed as a reaction to British colonialism in India, which was believed by a group of prominent Indian scholars consisting of Rashid Ahmad Gangohi, Muhammad Yaqub Nanautawi, Shah Rafi al-Din, Sayyid Muhammad Abid, Zulfiqar Ali, Fadhl al-Rahman Usmani and Muhammad Qasim Nanotvi to be corrupting the Islamic religion.
Later he added a colored outer wrapper and a friction strip – consisting of two overlapping strips of cardboard coated with a small amount of explosive powder-that is inside all ordinary crackers-and joined together, which became known as a " snap "-because when the cracker is pulled apart the strips rub across each other setting off a chemical reaction that produces an audible bang.
Inert atmospheres consisting of gases such as argon, nitrogen, or helium are commonly used in chemical reaction chambers and in storage containers for air-sensitive or water-sensitive substances, to prevent unwanted side-reactions of these substances with air and water.
This type of reaction will occur between any baryon ( particle consisting of three quarks ) and any antibaryon ( consisting of three antiquarks ).
The products of this process are saturated hydrocarbons ; depending on the reaction conditions ( temperature, pressure, catalyst activity ) these products range from ethane, LPG to heavier hydrocarbons consisting mostly of isoparaffins.
Very often, a serious acute reaction after the infusion ( 1 to 3 hours later ) is noted, consisting of high fever, shaking chills, hypotension, anorexia, nausea, vomiting, headache, dyspnea and tachypnea, drowsiness, and generalized weakness.
The AIP implemented on the S-80 class of the Spanish Navy is based on a bioethanol-processor ( provided by Hynergreen from Abengoa ) consisting of a reaction chamber and several intermediate Coprox reactors, that will transform the BioEtOH in high purity hydrogen.
Despite his unfamiliarity with frontier conditions and the methods of Indian fighting, Fetterman took command of a composite reaction force consisting of the former battalion quartermaster, Captain Frederick Brown, 2nd Lt. George Grummond, 49 enlisted troops of the 18th Infantry, 27 men of the 2nd Cavalry, and 2 civilian scouts, ironically totaling 80 men.
As the body attempts to clean up these wear particles ( typically consisting of plastic or metal ) it triggers an autoimmune reaction which causes resorption of living bone tissue.
Industrial Application-An enantioselective aldol addition product can be obtained in asymmetric synthesis by reaction of benzaldehyde with nitromethane and the a catalyst system consisting of a zinc triflate salt / the base diisopropylethylamine ( DIPEA ) and as chiral ligand is the N-methyl derivative of (+)- ephedrine ( NME ).
A cytokine storm, or hypercytokinemia is a potentially fatal immune reaction consisting of a positive feedback loop between cytokines and immune cells, with highly elevated levels of various cytokines.
A quick reaction force finally launched, consisting of two MH-47 Special Operations Aircraft of the 160th, two UH-60 conventional Army aviation Black Hawk helicopters, and two AH-64 Apache attack helicopters.
The reaction to this was an alliance consisting of the Confederate States, the British Empire, France, and Russia.
reaction and histone
In order for the reaction to proceed, S-Adenosyl methionine ( SAM ) and the lysine residue of the substrate histone tail must first be bound and properly oriented in the catalytic pocket of the SET domain.
reaction and naked
Her old friend Sally Seton, whom Clarissa admires dearly, is remembered as a great independent woman: She smoked cigars, once ran down a corridor naked to fetch her sponge-bag and made bold, unladylike statements to get a reaction from people.
The reaction of various elves as the team attempts to strip them naked is a primary basis for much of the show's humour, and are more ridiculous than risqué.
When he leaves, Quagmire shows up and tells Peter that he is returning his lawn mower, and asks for a towel, presumably in reaction to seeing Lois naked.
reaction and DNA
Nucleic acid amplification tests ( NAAT ), such as polymerase chain reaction ( PCR ), transcription mediated amplification ( TMA ), and the DNA strand displacement amplification ( SDA ) now are the mainstays.
In genetics, complementary DNA ( cDNA ) is DNA synthesized from a messenger RNA ( mRNA ) template in a reaction catalyzed by the enzyme reverse transcriptase and the enzyme DNA polymerase.
* Polymerase chain reaction, a technique used in molecular biology to amplify ( make many copies of ) a piece of DNA by in vitro enzymatic replication using a DNA polymerase.
Since blunt-ended DNA fragments have no cohesive ends to anneal, the melting temperature is not a factor to consider within the normal temperature range of the ligation reaction.
The polymerase chain reaction ( PCR ), a common laboratory technique, employs such artificial synthesis in a cyclic manner to amplify a specific target DNA fragment from a pool of DNA.
Typically, to generate the name of an enzyme, the suffix-ase is added to the name of its substrate ( e. g., lactase is the enzyme that cleaves lactose ) or the type of reaction ( e. g., DNA polymerase forms DNA polymers ).
Polymerase chain reaction is a method for selective replication of specific DNA and RNA sequences in the test tube.
Mullis worked as a DNA chemist at Cetus for seven years ; it was there, in 1983, that Mullis invented his prize-winning improvements to the polymerase chain reaction.
Current techniques for site-specific mutation commonly involve using mutagenic oligonucleotides in a primer extension reaction with DNA polymerase.
The polymerase chain reaction ( PCR ) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified.
Most PCR methods typically amplify DNA fragments of up to ~ 10 kilo base pairs ( kb ), although some techniques allow for amplification of fragments up to 40 kb in size. The reaction produces a limited amount of final amplified product that is governed by the available reagents in the reaction and the feedback-inhibition of the reaction products.
reaction and template
The main materials used in polymerase chain reaction are DNA nucleotides, template DNA, primers and Taq polymerase.
DNA nucleotides are the base for the new DNA, the template DNA is the specific sequence being amplified, primers are complementary nucleotides that can go on either side of the template DNA, and Taq polymerase is a heat stable enzyme that jump-starts the production of new DNA at the high temperatures needed for reaction.
A template is created by causing an ionic reaction at an electrolyte-metal interface which results in the spontaneous assembly of nanostructures, including quantum dots, onto the metal which is then used as a mask for mesa-etching these nanostructures on a chosen substrate.
The template DNA is immobile, and solutions of A, C, G, and T nucleotides are sequentially added and removed from the reaction.
5 ' RACE-PCR begins using mRNA as a template for a first round of cDNA synthesis ( or reverse transcription ) reaction using an anti-sense ( reverse ) oligonucleotide primer that recognizes a known sequence in the gene of interest ; the primer is called a gene specific primer ( GSP ), and it copies the mRNA template in the 3 ' to the 5 ' direction to generate a specific single-stranded cDNA product.
* PCR is an enzymatic reaction, therefore the quality and concentration of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome.
In both systems a template grows at expenses of a substrate in a cyclic reaction, combining growing and multiplication of the template units.
reaction and can
The most reaction can achieve is stasis, and a stasis that can be maintained only by the expenditure of an effort which ultimately exhausts itself.
Blowing can be either one of two types -- carbon dioxide gas generated by the reaction of water on the polyisocyanate or mechanical blowing through the use of a low-boiling liquid such as a fluorinated hydrocarbon.
The second reaction can be described using either theory.
Note that the acid can be the charged species and the conjugate base can be neutral in which case the generalized reaction scheme could be written as HA < sup >+</ sup > H < sup >+</ sup > + A.
This results in a characteristic type of irregular, uncoordinated movement that can manifest itself in many possible ways, such as asthenia, asynergy, delayed reaction time, and dyschronometria.
Unusual thermal Diels-Alder reactivity of arenes can be found in the Wagner-Jauregg reaction.
He showed that, although matter can change its state in a chemical reaction, the total mass of matter is the same at the end as at the beginning of every chemical change.
This can be caused by an autoimmune reaction, by certain infections or by some other rarer causes.
This reaction can be used to determine the position of a double bond in an unknown alkene.
Alkynes can be prepared from aldehydes using the Corey-Fuchs reaction and from aldehydes or ketones by the Seyferth – Gilbert homologation.
A severe allergic reaction that rarely can affect the bowel wall and secondarily cause abdominal pain can occur.
Only a proper, artist-grade acrylic gesso should be used to prime canvas in preparation for painting with acrylic ( however acrylic paint can be applied to raw canvas if so desired without any negative effect or chemical reaction as would be the case with oils ).
When the chain reaction occurs in a cell, it can cause damage or death to the cell.
The reverse reaction can also occur, using a molecule of water to split up a disaccharide and break the glycosidic bond ; this is termed hydrolysis.
It can be an adverse reaction to prescribed medication.
This can also be deduced from the equilibrium constant expression for the reaction:
A simple example can be seen in the combustion of hydrogen and oxygen, which is a commonly used reaction in rocket engines:
Solid materials that can sustain a smoldering reaction include coal, cellulose, wood, cotton, tobacco, peat, duff, humus, synthetic foams, charring polymers including polyurethane foam, and dust.
Energy that can be released ( or absorbed ) because of a reaction between a set of chemical substances is equal to the difference between the energy content of the products and the reactants.
* Computational studies can be used to predict the possibility of so far entirely unknown molecules or to explore reaction mechanisms that are not readily studied by experimental means.
Such a surface can be used for reaction dynamics.
Although it was originally presented in reaction to the statements of artificial intelligence researchers, it is not an argument against the goals of AI research, because it does not limit the amount of intelligence a machine can display.
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