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After and 30
After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.
After drying the sections under the fan, fixing in acetone, and drying at 37-degrees as in the indirect method, the sections were treated with conjugated Af or Af ( undiluted unless mentioned otherwise ) for 5 - 30 minutes.
" After much bargaining, the famine-stricken citizens agreed to pay a ransom of 5, 000 pounds of gold, 30, 000 pounds of silver, 4, 000 silken tunics, 3, 000 hides dyed scarlet, and 3, 000 pounds of pepper.
After his brother's death on 30 September / November 1204, Andrew took over the government of the kingdom as his nephew's tutor and he also seized the money his brother had deposited on behalf of the child Ladislaus.
After three years of searching, on December 30, 1907, Spalding received a letter that ( erroneously ) declared baseball to be the invention of Abner Doubleday.
After a recent increase in profits by 60 %, the company refused to raise worker's pay by more than 30 %.
After Mark Antony's death, Cleopatra eluded the vigilance of her guard Epaphroditus and committed suicide, on 12 August 30 BC.
After premiering on September 30, 1982, it was nearly canceled during its first season when it ranked last in ratings for its premiere ( 74th out of 77 shows ).
After the trade, however, Colavito hit over 30 home runs four times and made three All-Star teams for Detroit and Kansas City before returning to Cleveland in.
After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.
After losing each of the first two games by two runs, the Rockies opened up a 5 – 2 lead against Matt Cain in the finale of the series on August 30.
After some reverses, UN and Congolese government forces succeeded in recapturing the breakaway provinces of South Kasai on 30 December 1961, and Katanga on 15 January 1963.
After several script rewrites and changes, including a second title change to Friends Like Us, the series was finally named Friends and premiered on NBC's coveted Thursday 8: 30 pm timeslot.
After dating for nine months, the couple were married on October 30, 1943.
After a heated debate, the President of the Volkskammer, Sabine Bergmann-Pohl, announced the results at 2: 30 am on 23 August:
After almost 30 years of its release, Gaynor continues to ride the success of " I Will Survive ", touring the country and the world over and performing her signature song on dozens of TV shows.
After U. S. President Barack Obama announced the deployment of another 30, 000 soldiers in 2010 for a period of two years, Der Spiegel published images of the US soldiers who killed unarmed Afghan civilians.
After a medical examination three days later, Richard was told to rest his arm and he collapsed during a July 30 workout.
After Napoleon imposed the Convention of Artlenburg ( Convention of the Elbe ) on July 5, 1803, about 30, 000 French soldiers occupied Hanover.
After repeated negotiations between the Basel Council and Bohemia, a Bohemian – Moravian state assembly in Prague accepted the " Compacta " of Prague on 30 November 1433.
After about 30 years, the Japanese developed scale tuning and semitone harmonicas to be able to perform Japanese folk songs.
After the vote of the Partition Plan for Palestine on 30 November 1947, civil war broke out in Palestine.
* 1813 – War of 1812: After learning of American plans for a surprise attack on Beaver Dams in Ontario, Laura Secord sets out on a 30 kilometer journey on foot to warn Lieutenant James FitzGibbon.
After their joint suicide in 30 BCE, Octavian assumed control of the Roman Empire and granted Herod free rein over Jericho, as part of the new Herodian domain.

After and years
After he had spent the first three years in New York as associate conductor, at Toscanini's invitation, of the NBC Orchestra, he made numerous guest appearances throughout the United States and Latin America.
After only eighteen years of non-interference, there were already indications of melioration, though `` in a slight degree '', to be sure.
( After graduation from the University of Chicago, Paula taught for two years in the normal school at Valley City, North Dakota, then two years at Princeton ( Illinois ) Township High School.
After more years of concentrated effort, Roy and his brother Max finally established a thriving family business at the old stand.
After being located for some years in the Village at the Equinox Pharmacy under the supervision of Mrs. Harry Mercier, it is presently located in the Hill and Dale Shop, Manchester Center.
After many years and many interruptions he was able to finish the canopy fresco, and slightly less than half the frieze, beginning with the Liberty group opposite the East door, and ending with William Penn, all but one leg, when a tragic accident ended his career.
After 1895 the number increases, and in the next thirty years there is only one year for which there is no dated poem -- 1903, when Hardy was at work on The Dynasts.
After being a lawyer for twenty-five years he started studying for the ministry.
After years of digging, nights and weekends, he put together the big, profusely illustrated book, Of Garryowen And Glory, which is probably the most complete history of any military unit.
After her father's death, Lucy and her youngest sister lived for a few years with Winslow in Washington, D.C..
After 18 years in the personnel office, she has taken a disability pension on advice of her doctors.
His teacher was his mother, who supervised him for several years until she became terminally ill. After Hillside, he was educated at Eton College.
* After the Development of Agriculture, A. D. A., system of counting years, like A. D. and A. M.
After four years of warfare, mostly within the Southern states, the Confederacy surrendered and slavery was abolished everywhere in the nation.
After years of working on numerous films as an assistant director and scriptwriter, he made his debut as a director in 1943, during World War II with the popular action film Sanshiro Sugata ( a. k. a. Judo Saga ).
After four years of war-torn London, Christie hoped she can return some day to Syria, which she described as " gentle fertile country and its simple people, who know how to laugh and how to enjoy life ; who are idle and gay, and who have dignity, good manners, and a great sense of humor, and to whom death is not terrible.
After 25 years of parapsychological research, Susan Blackmore came to the conclusion that there is no empirical evidence for an afterlife.
After his government's defeat, Mackenzie remained Leader of the Opposition for another two years, until 1880.
After his full sister Tamar was raped by Amnon, their half-brother and David's eldest son, Absalom waited two years and avenged her by sending his servants to murder Amnon at a feast to which he had invited all the king's sons.
After this deed he fled to Talmai, the king of Geshur () ( see also or ), his maternal grandfather, and it was not until three years later that he was fully reinstated in his father's favour and finally returned to Jerusalem.
After four years he decided to declare himself king, sleeping with his father's concubines, then raised a revolt at Hebron, the former capital.
After several years of outdoor trading, the curbstone brokers moved indoors in 1921 to a building on Greenwich Street in Lower Manhattan.
After a brief but fierce storm sent up against the group at Juno's request, Aeneas and his fleet made landfall at Carthage after six years of wanderings.
The Kingdom of the Visigoths under Alaric II. After a few years, however, Clovis violated the peace treaty negotiated in 502.

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