After the factors just described have been computed, they are applied to the following formula: Af

After titration of the liberated Af with Af, aliquots of the aqueous and of the organic phase were counted in a solution-type Geiger tube.

After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.

After drying the sections under the fan, fixing in acetone, and drying at 37-degrees as in the indirect method, the sections were treated with conjugated Af or Af ( undiluted unless mentioned otherwise ) for 5 - 30 minutes.

After passing Af through DEAE-cellulose, the titer of antibodies to WTV in the specific fraction was 1: 4 of the titer before such passage ( precipitin ring tests by R. F. Whitcomb ) ; ;

After the first two murders, the warning notes were rarely ignored.

After only eighteen years of non-interference, there were already indications of melioration, though `` in a slight degree '', to be sure.

After a sort of death march during four days without food, Helion and his comrades were shipped by cattle-car to a labor camp at an estate farm in East Germany.

After we got a script and the spots for the songs were blocked out, we'd get together for an hour or so every day.

After a pause, during which he studied Scotty's face as if Scotty were not there and could not study him too, Mr. McKinley would ask the same questions he had asked downstairs.

After the Juniors were welcomed and congratulated for qualifying for the Finals of the Junior Class, Mrs. William H. Long, Jr. was introduced as the first speaker.

After all, if you were going to set up a workshop you had to have the proper equipment and that was that.

After the 42 figures had been drawn like Figures 5 and 6, classifications of the onset ages and completion ages were summarized from them.

After blotting out most of the liquid around the sections, the latter were mounted in buffered glycerine ( 7 parts glycerine to 3 parts of PBS ).

After every session, the check marks were totaled up and graphed, and in this way the supervisor's progress was charted.

After a while we were perhaps less surprised, but still puzzled, when a friendly discussion would suddenly jump the track into the most irrelevant and illogical comparisons.

After the first two were blacked out, the third light was abandoned by a terrified Italian crew, who left their light to shine for nine minutes like an unerring homing beacon until British MP's shot it out.

After a flood of protests, they were reinstated at the beginning of 1953.

After an earthquake in Japan in 1793 people on the coast at Tugaru were so terrified by the extraordinary ebbing of the sea that they scurried to higher ground.

After he had proceeded a few feet, he paused and turned up the cuffs of his trousers, which were already damp and mud-caked.

After a time, it occurred to her that her thoughts were not worth the trouble.

After it allotted shares to 41 underwriters and 52 selling group members from coast to coast there were not many shares for anyone.

After this time, inscriptions on the Han bronze mirrors, as well as other writings, emphasized the desirability of keeping one's self at the center of the universe, where cosmic forces were strongest.

After returning to the United States and raising the money, he discovered on getting back to Japan that the hours were no longer available.

After the Revolution of 1917, views expressed by anthropologists in the USSR, and later the Soviet Bloc countries, were highly shaped by the requirement to conform to Marxist theories of social evolution.

After they each performed, both were deemed equal until Apollo decreed they play and sing at the same time.

" After the war, nationalists, especially those who had been active in the Continental Army, complained that the Articles were too weak for an effective government.

After a long debate in the House, a second attempt passed Congress on January 13, 1865, and was sent to the state legislatures for ratification.

After the collapse of the Sanhaja union, Awdagust passed over to the Ghana empire and the trans-Saharan routes were taken over by the Zenata Maghrawa of Sijilmassa.

After the death of Fulk in a hunting accident in 1143, the throne passed jointly to Melisende and Amalric's older brother Baldwin III, who was still only 13 years old.

After March it began to pass northwards, a motion quite apparent by the middle of April ; in June it passed at the same distance from the zenith as it did in December ; and in September it passed through its most northerly position, the extreme range from north to south, i. e. the angle between the March and September positions, being 40 ″.

After two previous failures, voters in the city passed a ballot measure in 2000 authorizing a bond measure for construction of a new library to replace the city's Carnegie library, damaged during the Loma Prieta earthquake.

After a period of fifty years has passed, Beowulf defeats a dragon, but is fatally wounded in the battle.

After years of extensive lobbying for federal dollars, a 1987 public works bill appropriating funding for the Big Dig was passed by U. S. Congress, but it was subsequently vetoed by President Ronald Reagan as being too expensive.

After a brief Conservative interlude ( during which the Second Reform Act was passed by agreement between the parties ), Gladstone won a huge victory at the 1868 election and formed the first Liberal government.

After the Empire passed, use of concrete became scarce until the technology was re-pioneered in the mid-18th century.

After World War II and the implementation of the Oder-Neisse line in 1945, the town passed to the Republic of Poland.

After the 1974 federal election and subsequent Joint Sitting of parliament, legislation was passed to create a unicameral Legislative Assembly, as well as members with full voting rights at a federal level.

After Domitian's assassination, the senators of Rome rushed to the Senate house, where they immediately passed a motion condemning his memory to oblivion.

After a month had passed with little progress in the case, police continued to focus their investigation upon Echols, interrogating him more frequently than any other person ; however, they claimed he was not regarded as a direct suspect but a source of information.

After the Immigration Act of 1924 was passed, which greatly restricted immigration and allowed processing at overseas embassies, the only immigrants to pass through the station were displaced persons or war refugees.

After less than a year had passed in Silsbee's office, Wright learned that the Chicago firm of Adler & Sullivan was " looking for someone to make the finish drawings for the interior of the

After a very heated national debate, legislation was passed in 1985 that gave women an equal right to decide what surname or surnames they and their children would use.

After the 1986 amendments strengthening the Act were passed ( see below ), the Act was used primarily against defense contractors.

When he unexpectedly died of a heart attack in January 1975, his share of TSR passed to his wife, a woman whom Gygax characterized as " less than personable ... After Don died she dumped all the Tactical Studies Rules materials off on my front porch.

After prolonged years of struggle, in 1834 the Evangelicals gained control of the General Assembly and passed the Veto Act, which allowed congregations to reject unwanted " intrusive " presentations to livings by patrons.

After having been passed over for the All-Star team in 1935 and being left on the bench for the 1937 game, Greenberg refused to participate in the 1938 contest.

After passing the initial aptitude test, he was to undertake a month-long course ; if he passed that, he would begin full flight training in early 2009.

After telling the audience " I shall now read to you the scroll of the Establishment of the State, which has passed its first reading by the National Council ", Ben-Gurion proceeded to read out the declaration, taking 16 minutes, ending with the words " Let us accept the Foundation Scroll of the Jewish State by rising " and calling on Rabbi Fishman to recite the Shehecheyanu blessing.

After the perpetrator of President Reagan's assassination attempt was found not guilty by reason of insanity, Congress passed the Insanity Defense Reform Act of 1984.