After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.

After drying the sections under the fan, fixing in acetone, and drying at 37-degrees as in the indirect method, the sections were treated with conjugated Af or Af ( undiluted unless mentioned otherwise ) for 5 - 30 minutes.

" After much bargaining, the famine-stricken citizens agreed to pay a ransom of 5, 000 pounds of gold, 30, 000 pounds of silver, 4, 000 silken tunics, 3, 000 hides dyed scarlet, and 3, 000 pounds of pepper.

After his brother's death on 30 September / November 1204, Andrew took over the government of the kingdom as his nephew's tutor and he also seized the money his brother had deposited on behalf of the child Ladislaus.

After three years of searching, on December 30, 1907, Spalding received a letter that ( erroneously ) declared baseball to be the invention of Abner Doubleday.

After a recent increase in profits by 60 %, the company refused to raise worker's pay by more than 30 %.

After Mark Antony's death, Cleopatra eluded the vigilance of her guard Epaphroditus and committed suicide, on 12 August 30 BC.

After premiering on September 30, 1982, it was nearly canceled during its first season when it ranked last in ratings for its premiere ( 74th out of 77 shows ).

After the trade, however, Colavito hit over 30 home runs four times and made three All-Star teams for Detroit and Kansas City before returning to Cleveland in.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After losing each of the first two games by two runs, the Rockies opened up a 5 – 2 lead against Matt Cain in the finale of the series on August 30.

After some reverses, UN and Congolese government forces succeeded in recapturing the breakaway provinces of South Kasai on 30 December 1961, and Katanga on 15 January 1963.

After several script rewrites and changes, including a second title change to Friends Like Us, the series was finally named Friends and premiered on NBC's coveted Thursday 8: 30 pm timeslot.

After dating for nine months, the couple were married on October 30, 1943.

After a heated debate, the President of the Volkskammer, Sabine Bergmann-Pohl, announced the results at 2: 30 am on 23 August:

After almost 30 years of its release, Gaynor continues to ride the success of " I Will Survive ", touring the country and the world over and performing her signature song on dozens of TV shows.

After U. S. President Barack Obama announced the deployment of another 30, 000 soldiers in 2010 for a period of two years, Der Spiegel published images of the US soldiers who killed unarmed Afghan civilians.

After a medical examination three days later, Richard was told to rest his arm and he collapsed during a July 30 workout.

After Napoleon imposed the Convention of Artlenburg ( Convention of the Elbe ) on July 5, 1803, about 30, 000 French soldiers occupied Hanover.

After repeated negotiations between the Basel Council and Bohemia, a Bohemian – Moravian state assembly in Prague accepted the " Compacta " of Prague on 30 November 1433.

After about 30 years, the Japanese developed scale tuning and semitone harmonicas to be able to perform Japanese folk songs.

After the vote of the Partition Plan for Palestine on 30 November 1947, civil war broke out in Palestine.

* 1813 – War of 1812: After learning of American plans for a surprise attack on Beaver Dams in Ontario, Laura Secord sets out on a 30 kilometer journey on foot to warn Lieutenant James FitzGibbon.

After their joint suicide in 30 BCE, Octavian assumed control of the Roman Empire and granted Herod free rein over Jericho, as part of the new Herodian domain.

After the decline of the Ilkhanate from 1335 – 1353, the Mongol Empire's legacy in the region was the Uyghur Eretna Dynasty that was overthrown by Kadi Burhan al-Din in 1381.

* 1956 – After going bankrupt in 1955, the American broadcaster DuMont Television Network makes its final broadcast, a boxing match from St. Nicholas Arena in New York in the Boxing from St. Nicholas Arena series.

After what the MCC saw as the problems of the earlier professional and amateur series they decided to take control of organising tours themselves, and this led to the first MCC tour of Australia in 1903 – 04.

After winning the First Test by an innings after being controversially sent in by Hutton, Australia lost its way and England took a hat-trick of victories to win the series 3 – 1.

After Australia took a 1 – 0 lead in the first two Tests, Botham was forced to resign or was sacked ( depending on the source ).

After re-establishing its credibility in 1989, Australia underlined its superiority with victories in the 1990 – 91, 1993, 1994 – 95, 1997, 1998 – 99, 2001 and 2002 – 03 series, all by convincing margins.

After 1890 came philosopher Josiah Royce ( 1855 – 1916 ), botanist Liberty Hyde Bailey ( 1858 – 1954 ), the Southern Agrarians of the 1920s and 1930s, novelist John Steinbeck ( 1902 – 1968 ), historian A. Whitney Griswold ( 1906 – 1963 ), environmentalist Aldo Leopold ( 1887 – 1948 ), Ralph Borsodi ( 1886 – 1977 ), and present-day authors Wendell Berry ( b. 1934 ), Gene Logsdon ( b. 1932 ), Paul Thompson, and Allan C. Carlson ( b. 1949 ).

* 1967 – After refusing induction into the United States Army the day before ( citing religious reasons ), Muhammad Ali is stripped of his boxing title.

After Roman times, Einhard mentions that in 765 – 6 Pippin the Younger spent both Christmas and Easter at Aquis villa (""), which must have been sufficiently equipped to support the royal household for several months.

" After a vigorous debate, a formal vote for impeachment was held in the House of Representatives on December 5, 1867, and failed, 57 – 108.

After being widowed in 1327, Alfonso married in February 1329 Eleanor of Castile ( 1308 – 1359 ), who was betrothed to his brother James, who had refused to consummate the marriage.

* 1945 – World War II: Führerbunker: After learning that Soviet forces have taken Eberswalde without a fight, Adolf Hitler admits defeat in his underground bunker and states that suicide is his only recourse.

* 1056 – After a sudden illness a few days previously, Byzantine Empress Theodora dies childless, thus ending the Macedonian dynasty.

* 1980 – After two weeks of nationwide strikes, the Polish government was forced to sign the Gdańsk Agreement, allowing for the creation of the trade union Solidarity.

* 1930 – After the mysterious death of Empress Zewditu, Haile Selassie is proclaimed emperor of Ethiopia.

* 1849 – After a month-long siege, Venice, which had declared itself independent as the Republic of San Marco, surrenders to Austria.

* 1896 – Philippine Revolution: After Spanish victory in the Battle of San Juan del Monte, eight provinces in the Philippines are declared under martial law by the Spanish Governor-General Ramón Blanco y Erenas.