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After and blotting
After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.
After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining ( for example, silver staining of proteins ), autoradiographic visualization of radioactive labelled molecules ( performed before the blot ), or specific labelling of some proteins or nucleic acids.
After washing, the precipitated protein ( s ) are eluted and analyzed by gel electrophoresis, mass spectrometry, western blotting, or any number of other methods for identifying constituents in the complex.

After and out
After walking out to his corral that morning, he'd been amazed to see the dust puff up in front of his feet.
After he had finished the first two volumes of his Lincoln, Sandburg went to work assembling a book of songs out of hobo and childhood days and from the memory of songs others had taught him.
After we got a script and the spots for the songs were blocked out, we'd get together for an hour or so every day.
After baptism Jesus came up out of the water at once, and at that moment heaven opened ; ;
After a nuclear blast, one bureaucrat suggested in those halcyon days, about all you had to do was haul out the broom and sweep off your sidewalks and roof.
After Mr. Jack drove away, Winston went on looking out the window.
After the family has settled in the shelter, the housekeeping rules should be spelled out by the adult in charge.
After figuring out how to regulate the barrels so that they shot to the same point of impact, we fired this little 20-inch-barrel job on my home range and in Marlin's underground test gallery.
After the meal, he and his guests went out to inspect the rig ; ;
After an unspeakable siege, lasting the better part of two months, it was announced that the studio `` owed '' the government a tax debt in excess of eight million dollars while I, who had always remained aloof from such iniquitous practices as paying taxes on the salary I had earned and the little I legally inherited as Morris' helpless relict, was `` stung '' with a personal bill of such astronomical proportions as to `` wipe out '' all but a fraction of my poor, hard-come-by savings.
After the first two were blacked out, the third light was abandoned by a terrified Italian crew, who left their light to shine for nine minutes like an unerring homing beacon until British MP's shot it out.
After all, the henpecked husband with his shrewish wife is a comic figure of long standing, in literature and on the stage, as Dr. Schillinger points out.
After he'd got out fresh liver for Bast, he paused to look at her crouched daintily over her dish.
After Al Paschal gruonded out, Jay Cooke walked and Jim McDaniel singled home McAuliffe.
After playing a splendid first nine holes in 34 -- two strokes under par -- on this fifth and final day of the tournament ( Sunday's fourth round had been washed out by a violent rainstorm when it was only half completed ), Player's game rapidly fell to pieces.
After the sad impatient moment, waiting for comfort which could not come, she slipped out of bed and went to the open window.
After a short time, both George and Donald joined the class with me so they wouldn't feel lonely, and we used to hang a sign on the door of the Brush-off reading out to work.
After the Battle of Gettysburg, Lincoln clearly understood that his military decisions would be more effectively carried out by conveying his orders through his War Secretary or his general-in-chief on to his generals, who resented his civilian interference with their own plans.
After the Civil War, Doubleday mustered out of the volunteer service on August 24, 1865, reverted to the rank of lieutenant colonel, and became the colonel of the 35th U. S. Infantry in September 1867.
After seven days and nights in agony, Alcmene stretched out her arms and called upon Lucina, the goddess of childbirth ( the Roman equivalent of Eileithyia ).
After the pair donned and pressurized their spacesuits and depressurized the Lunar Module cabin, John Young climbed out onto the " porch " of the LM, a small platform above the ladder.
After completing more tasks at the ALSEP, Young and Duke returned to the LM to close out the moonwalk.

After and most
After a year in a studio on Sheridan Square, having married an American girl who was a native of Virginia, Helion moved to a village in the Blue Ridge mountains, where he produced some of the most imposing of his abstract canvases.
After the Griffin-Byrd political troup has completed the circuit in November in the name of a Pre-Legislative Forum, this is going to be the most politically oriented Legislature in history.
After declaring, in an article last month in Frontier Magazine, that the Russian testing `` carries with it the possibility of the most tragic consequences of any action in the history of the world '', he gave this estimate of the biologic and genetic consequences if the new Soviet shots totaled 200 megatons:
-- After 52 rainless days, moisture finally came to Del Mar, resulting in but one workout during the week for most of the horses, and leaving us with less than half our total average rainfall during the season.
After the first month, rates are considerably less, averaging only about $60 a month for most 4- and 5-passenger models.
After a while we were perhaps less surprised, but still puzzled, when a friendly discussion would suddenly jump the track into the most irrelevant and illogical comparisons.
After years of digging, nights and weekends, he put together the big, profusely illustrated book, Of Garryowen And Glory, which is probably the most complete history of any military unit.
After all, his wife had written most of his letters for him in those first lean days of Partlow Products.
After the war, the critically acclaimed Drunken Angel ( 1948 ), in which Kurosawa cast then-unknown actor Toshiro Mifune in a starring role, cemented the director's reputation as one of the most important young filmmakers in Japan.
After World War II, most of the Ainu living in Sakhalin were deported to Japan.
After Achilles, Ajax is the most valuable warrior in Agamemnon's army ( along with Diomedes ), though he is not as cunning as Nestor, Diomedes, Idomeneus, or Odysseus, he is much more powerful and just as intelligent.
After the election Johnson was most anxious to complete the re-establishment of civil government in Tennessee ; Union forces brought the war to an end in that state with their victory in the Battle of Nashville in December.
After this battle and the loss of one of Portugal's most remarkable infantes, the Duke of Braganza became the de facto ruler of the country.
After the American Civil War, the settlement of the western territories by migrants from the east led to dialect mixing and levelling, so that regional dialects are most strongly differentiated in the eastern parts of the country that were settled earlier.
After the first battle between two ironclads took place in 1862 during the American Civil War, it became clear that the ironclad had replaced the unarmoured line-of-battle ship as the most powerful warship afloat.
After widespread adoption of the COX-2 inhibitors, it was discovered that most of the drugs in this class increased the risk of cardiovascular events by 40 % on average.
After the accession of the latter to the imperial purple he invited Aedesius to continue his instructions, but the declining strength of the sage being unequal to the task, two of his most learned disciples, Chrysanthius and the aforementioned Eusebius, were by his own desire appointed to supply his place.
After the protonotaries left the sketching of the minutes to the abbreviators, those de Parco majori, who ranked as prelates, were the most important officers of the apostolic chancery.
After a long and difficult fight “ Abd ar-Rahman obtained a most complete victory, and the field was strewn with the bodies of the enemy ”.
After March it began to pass northwards, a motion quite apparent by the middle of April ; in June it passed at the same distance from the zenith as it did in December ; and in September it passed through its most northerly position, the extreme range from north to south, i. e. the angle between the March and September positions, being 40 ″.
After that point, however, most cellular companies were eager to shut down AMPS and use the remaining channels for digital services.
After a period of confusion, Demetrius's son Antigonus II Gonatas was able to establish the family's control over the old Kingdom of Macedon, as well as over most of the Greek city-states, by 276 BC.
After conquering Edirne ( Adrianople ) in 1365 the Ottomans turned it into a joint capital city for governing their European realms, but Bursa remained the most important Anatolian administrative and commercial center even after it lost its status as the sole Ottoman capital.

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