After these treatments the conjugate did not stain healthy or crown gall sweet clover tissues or stained them a very faint green which was easily distinguishable from the bright yellow-green specific staining.

After Af and Af were passed through Dowex-2-chloride twice and treated twice with healthy sweet clover tissue powder, nonspecific staining was greatly reduced but a disturbing amount of such staining was still present.

After staining, the monolayer is viewed under a microscope using magnification up to 1000x.

After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining ( for example, silver staining of proteins ), autoradiographic visualization of radioactive labelled molecules ( performed before the blot ), or specific labelling of some proteins or nucleic acids.

After staining, unbound dye is removed by washing five times with 1 % acetic acid and the plates are air dried.

After separation of red cell membranes by SDS-polyacrylamide gel electrophoresis and staining with periodic acid-Schiff staining ( PAS ) four glycophorins have been identified.

After immunohistochemical staining of the target antigen, a second stain is often applied to provide contrast that helps the primary stain stand out.

After James Prescott Joule had determined the mechanical equivalent of heat, Lord Kelvin approached the question from an entirely different point of view, and in 1848 devised a scale of absolute temperature which was independent of the properties of any particular substance and was based solely on the fundamental laws of thermodynamics.

After his conversion to Christianity and his baptism in 387, Augustine developed his own approach to philosophy and theology, accommodating a variety of methods and different perspectives.

Four different mixes were done, with one appearing on their retrospective album, Fifteen Years After.

After three years of consistent use across different platforms, the BBC began to drop the BBCi brand gradually ; on 6 May 2004, the BBC website was renamed bbc. co. uk, after the main URL used to access the site.

After a month-long recount and several appeals from different parties, Arias was declared the official winner with 40. 9 % of the votes against 39. 8 % for Solís.

After a tie in the 1969 voting, the process was changed, in which each writer was to vote for three different pitchers: the first-place vote received five points, the second-place vote received three points, and the third-place vote received one point.

After the captains of the two teams met they quickly realized that the games each school played were still different.

After Paul's departure, the churches were led astray from Paul's faith / trust centered teachings by individuals proposing " another gospel " ( which centered around salvation through the Mosaic law, so-called legalism ), whom Paul saw as preaching a " different gospel " from what Paul had taught.

After a few more raids across the Rhine, which resulted in the recovery of two of the three legion's eagles lost in 9, Germanicus was recalled to Rome and informed by Tiberius that he would be given a triumph and reassigned to a different command.

After moving to a different domain, that service has since been discontinued.

After these lines ended in extinction, the electors began to elect kings from different families so that the throne would not once again settle within a single dynasty.

After the Shona speaking people moved in to the present day Zimbabwe many different dialects developed over time in the different parts of the country.

After surveying a number of different locations, Pei fell in love with a valley once used as an imperial garden and hunting preserve, known as Fragrant Hills.

After the troops based in different parts of British East and Central Africa territories were placed under a central command, the regiment born thereof was officially designated “ King's African Rifles ” on 1 January 1902.

After the independence of Pakistan, Karachi received a large number of refugees from all over India, whose influence is now evident in the city's different sub-cultures.

After that, ships like San Jeronimo, Los Reyes, Todos los Santos also visited the islands in different years.

After President Najibullah's resignation in 1992 and the end of Soviet support, the military dissolved into portions controlled by different warlord factions and the mujahideen took control over the government.

After the death of Clovis there were frequent clashes between different branches of the family, but when threatened by its neighbours the Merovingians presented a strong united front.

After Miller's death in 2007, scientists examining sealed vials preserved from the original experiments were able to show that there were actually well over 20 different amino acids produced in Miller's original experiments.

After a brief respite, Moore threw herself into a completely different genre.

After the immense success of SimCity, Maxis experimented with different genres.

After the files are uploaded, the uploader does not have any control over them ; the files are automatically copied to all Usenet providers, so there will be multiple copies of it spread over different geographical locations around the world.

: " 1 ... First then must be put the holy quaternion of the gospels ; following them the Acts of the Apostles ... the epistles of Paul ... the epistle of John ... the epistle of Peter ... After them is to be placed, if it really seem proper, the Book of Revelation, concerning which we shall give the different opinions at the proper time.

After Nichiren's death, his teachings were interpreted in different ways.

After binding dsRNA, this receptor activates the transcription factors IRF3 and NF-κB, which are important for initiating synthesis of many inflammatory proteins.

After arriving an endosome via the pathway described above, vesicles begin to form inside the endosome, taking with them the membrane proteins meant for degradation ; When the endosome either matures to become a lysosome or is united with one, the vesicles are completely degraded.

After the discovery of the structure of DNA in 1953 by Francis Crick and James D. Watson, Gamow attempted to solve the problem of how the order of the four different kinds of bases ( adenine, cytosine, thymine and guanine ) in DNA chains could control the synthesis of proteins from amino acids.

After a set amount of time ( usually a few hours, though this depends on the voltage applied across the gel ; protein migration occurs more quickly at higher voltages, but these results are typically less accurate than at those at lower voltages ) the proteins will have differentially migrated based on their size ; smaller proteins will have traveled farther down the gel, while larger ones will have remained closer to the point of origin.

After antigens initiate an immune response, these white blood cells send signals, composed of cytokines and other hormonal proteins, to the brain and neuroendocrine system.

After the 1970s, nucleic acid sequencing allowed molecular evolution to reach beyond proteins to highly conserved ribosomal RNA sequences, the foundation of a reconceptualization of the early history of life.

After returning to Switzerland, Wüthrich collabrated with among others nobel laureate Richard R. Ernst on developing the first 2 dimensional NMR experiments, and established the nuclear Overhauser effect as a convenient way of measuring distances within proteins.

After entering the chloroplast, the first targeting peptide is cleaved off by a protease processing imported proteins.

After binding to Glycyl-Prolyl-Prolyl-Prolyl-Prolyl-Prolyl-registers on tracker proteins, Profilin-ATP-actin is delivered (" loaded ") to the unclamped end of the other sub-filament, whereupon ATP within the already clamped terminal subunit of the other subfragment is hydrolyzed (" fired "), providing the energy needed to release that arm of the end-tracker, which then can bind another Profilin-ATP-actin to begin a new monomer-addition round.

After engulfment, foreign proteins ( the antigens ) are broken down into peptides inside dendritic cells and macrophages.

After completion of the first dimension the complexes are destroyed by applying the denaturing SDS-PAGE in the second dimension, where the proteins of which the complexes are composed of are separated by their mass.

After this extraction process soluble proteins will be in the solvent, and can be separated from cell membranes, DNA etc.

After the DNA is transcribed and mRNA is formed, there must be some sort of regulation on how much the mRNA is translated into proteins.

After removing the brains and properly preserving them, the group used various antibodies and antiserums to detect the presence of the proteins.

After expression of the gene product, the purification of the protein is required ; but since the vector is introduced to a host cell, the protein of interest should be purified from the proteins of the host cell.

After researchers in other laboratories were unable to replicate Spector's results, it was found that Spector had knowingly incorporated radioactive iodine into proteins rather than radioactive phosphate, allowing him to fabricate an imaginary regulatory cascade.

After successful infection of a keratinocyte, the virus expresses E1 and E2 proteins, which are for replicating and maintaining the viral DNA as a circular episome.

After protein folding in aqueous solution, hydrophobic amino acids usually form protected hydrophobic areas while hydrophilic amino acids interact with the molecules of solvation and allow proteins to form hydrogen bonds with the surrounding water molecules.

After interacting with the host cytoskeletal proteins, the bacterium develops an actin tail.

After infection of the host cell, the genome is translated in a cap-independent manner into a single polyprotein, which is subsequently processed by virus-encoded proteases into the structural capsid proteins and the nonstructural proteins, which are mainly involved in the replication of the virus.