After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After starting his writing career by writing for " true confession " style pulp magazines like True Story, van Vogt decided to switch to writing something he enjoyed, science fiction.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After the 42 figures had been drawn like Figures 5 and 6, classifications of the onset ages and completion ages were summarized from them.

After drying the sections under the fan, fixing in acetone, and drying at 37-degrees as in the indirect method, the sections were treated with conjugated Af or Af ( undiluted unless mentioned otherwise ) for 5 - 30 minutes.

" After much bargaining, the famine-stricken citizens agreed to pay a ransom of 5, 000 pounds of gold, 30, 000 pounds of silver, 4, 000 silken tunics, 3, 000 hides dyed scarlet, and 3, 000 pounds of pepper.

" After a vigorous debate, a formal vote for impeachment was held in the House of Representatives on December 5, 1867, and failed, 57 – 108.

After the ceremony, the Diamondbacks won the game against the San Diego Padres 6 – 5 after a walk-off home run by Chris Young, who also led the game off with a home run in the Bottom of the First Inning.

* 1858 – After the original Big Ben, a 14. 5 tonne bell for the Palace of Westminster had cracked during testing, it is recast into the current 13. 76 tonne bell by Whitechapel Bell Foundry.

After a first rupture, the annual bleeding risk may increase to more than 5 %.

* After crossing the Jordan River, the Israelites celebrated the Passover ( 5: 10 – 12 ) just as they did immediately before the Exodus ( Ex.

After 10 rounds, play 1 per round, the average house profit will be 10 x 1 x 5. 26 % = 0. 53.

After 10 rounds, the expected loss will be 10 x 1 x 5. 26 % = 0. 53.

After 5 years of researching grave sites, he concluded that " these mass graves contain the remains of 1, 386, 734 victims of execution ".

After quarterback Jay Cutler came down with an injury in 2011, where the Bears lost 5 straight games and was knocked out of playoff contention, his absence was bemoaned in a song parodying the hit Baby Come Back as a parody called Cutty Come Back.

After a brief July slump, the Indians caught fire in August, and cut a 15. 5 game deficit in the Central Division down to 1. 5 games.

After being beaten in Game 3 7 – 1, the Cubs lost Game 4 when Smith, with the game tied 5 – 5, allowed a game-winning home run to Garvey in the bottom of the ninth inning.

After losing each of the first two games by two runs, the Rockies opened up a 5 – 2 lead against Matt Cain in the finale of the series on August 30.

After 1990 the Mexican census has been taken every 5 years.

After 1935, it took place every 5 years until 1990.

After many months of negotiation, agreement was reached between Hydro Quebec and Rediffusion on February 28, 1950 for an initial 5 year period.

After his adoptive mother's death when he was 5, his father moved around the country seeking work.

In a December 5, 2007 article on Mutato Muzika, LA Weekly reported that " After touring sporadically over the past decade but not releasing any new material, Devo are spending December at Mutato trying to create an album ’ s worth of new material and contemplating a method of dispersal in the post-record-company world.

After another 10 minutes its amplitude would be only 2. 5 cm, then 1. 25 cm, etc.

After the severe economic troubles of the early 1990s, brought on by a drop in the vital fish catch and poor management of the economy, the Faroe Islands have come back in the last few years, with unemployment down to 5 % in mid-1998, and holding below 3 % since 2006, one of the lowest rates in Europe.

After ten weeks nearly 17. 5 million people had seen the film in France, Intouchables was the second most-seen French movie of all-time in France, and the third including foreign movies.