After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After leaving school at 17, Ayckbourn's career took several temporary jobs in various places before starting a temporary job at the Scarborough Library Theatre, where he was introduced to the artistic director, Stephen Joseph.

After the tumultuous and disappointing 2007 season, the Falcons made a number of moves, hiring a new General Manager and head coach, drafting a new starting quarterback, and signing a starting running back.

After rallying his troops near Schwennenbach – well beyond their starting point – Eugene prepared to launch a second attack, led by the second-line squadrons under the Duke of Württemberg-Teck.

After starting the 1954 campaign with a two-game split against the Tigers in Detroit, the Orioles returned to Baltimore on April 15 to a welcoming parade that wound through the streets of downtown, with an estimated 350, 000 spectators lining the route.

After the victory Boston's owner sold its star pitcher, Babe Ruth, to the New York Yankees to help funding of a Broadway play, and thus starting a tale of futility which would last 86 years, known as Curse of the Bambino.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After defeating, but not subjugating, the Kingdom of Scotland, he declared himself rightful heir to the French throne in 1338, starting what would be known as the Hundred Years ' War.

After passing his examinations in 1752, he soon progressed through the merchant navy ranks, starting with his promotion in that year to mate aboard the collier brig Friendship.

After various Korean border conflicts, war broke out with U. S .- allied South Korea in 1950, starting the Korean War.

After cutting out the hatchlings may lie in their eggshells for hours before starting to dig out of the nest.

After being sent on a lengthy tour of Europe starting 1927 – including London, Paris and towns in Germany – during which he demonstrated his invention to full audiences, Theremin found his way to the United States, arriving on 30 December 1927 with his first wife Katia.

After the 1997 death of Scott Krueger, a new member at the Phi Gamma Delta fraternity, MIT required all freshmen to live in the dormitory system starting in 2002.

After starting a collaboration on heart applications with Robert Weiss at Johns Hopkins, Bottomley returned to the university in 1994, as Russell Morgan Professor and Director of the MR Research Division.

After the introduction of the motel, auto camps continued in popularity through the Depression years and after World War II, their popularity finally starting to diminish with the construction of freeways and changes in consumer demands.

After starting 3 – 7 in 1994, the Giants won their final six games to finish 9 – 7 but missed the playoffs.

After starting 5 – 0 in the 2009 season, New York lost to the likewise undefeated New Orleans Saints at the Superdome 48 – 27, beginning a four game losing streak, in which they lost to the Arizona Cardinals 24 – 17, the San Diego Chargers 21 – 20 and the Philadelphia Eagles 40 – 17.

After La Russa retired as manager of the Cardinals in 2011, Smith became active in the organization again, starting with his stint as a special instructor for the team's 2012 spring training camp.

After everyone has made their decision, the replacement cards are dealt starting at the dealer's left as usual.

After reaggravating a hamstring injury during Game 3 of the 2007 ALDS, Joe Torre removed Roger Clemens from the team's starting rotation.

After starting the engine with a known setting that allowed it to idle, the air valve was opened until maximum engine speed was obtained.

After a while, Prokofiev's mother felt that the isolation in Sontsovka was restricting his further musical development, yet his parents hesitated over starting their son on a musical career at such an early age.

After the Welsh victory at the battle of Llwchwr in January 1136 and the successful ambush of Richard Fitz Gilbert de Clare in April, south Wales rose in rebellion, starting in east Glamorgan and rapidly spreading across the rest of south Wales during 1137.

After World War II, the British greatly reduced the use of the full stop and other punctuation points after abbreviations in at least semi-formal writing, while the Americans more readily kept such use until more recently, and still maintain it more than Britons.

After the last anointing, the Gospel Book is opened and placed with the writing down upon the head of the one who was anointed, and the senior priest reads the " Prayer of the Gospel ".

After the good experience of writing the screenplay for a film called Easy Wheels, Sam and his brother Ivan Raimi decided to co-write the film together.

After ABBA broke up in 1983, Andersson continued writing music with Ulvaeus.

After the death of the Buddha, attempts were made to gather his teachings and transmit them in a commonly agreed form, first orally, then also in writing ( the Tripiṭaka ).

After the death of Willy, Colette went to court to challenge her former husband's involvement in the writing, and subsequently had his name removed from the books.

After Anne's death Charlotte resumed writing as a way of dealing with her grief, and Shirley which deals with the themes of industrial unrest and the role of women in society was published in October 1849.

After Kuttner's death in 1958, Moore wrote almost no fiction and taught his writing course at the University of Southern California.

After the Tories fell from power with the death of Queen Anne, Defoe continued doing intelligence work for the Whig government, writing " Tory " pamphlets that actually undermined the Tory point of view.

After many letter writing campaigns demanding the removal of the strip were unsuccessful, conservatives changed their tactics, and instead of writing to newspaper editors, they began writing to one of the printers who prints the color Sunday comics.

After graduating with the Emerson Class of 1979, he took a job at the school teaching comedy writing classes and maintained the job for five years.

After some ten years of development, which Zamenhof spent translating literature into Esperanto as well as writing original prose and verse, the first book of Esperanto grammar was published in Warsaw in July 1887.

After a more thorough qualitative comparison of all three encyclopaedias, Kister recommended Collier's Encyclopedia as the superior encyclopaedia, primarily on the strength of its writing, balanced presentation and easy navigation.

After his death Frederick the Great, who several times needed Algarotti writing texts in Latin, sent in a text for a monument to his memory on the Campo Santo in Pisa, Italy.

After escaping from slavery, he became a leader of the abolitionist movement, gaining note for his dazzling oratory and incisive antislavery writing.

After the 1991 United Nations Conference on Environment and Development, the Earth Summit, Singer started writing and speaking out to cast doubt on the science.

After Brandeis denied the renewal of his teaching contract in 1965, Marcuse devoted the rest of his life to teaching, writing and giving lectures around the world.

After the Tripartite Pact was signed by Axis Powers Germany, Japan and Italy, in October 1940, Stalin traded letters with Ribbentrop, with Stalin writing about entering an agreement regarding a " permanent basis " for their " mutual interests.

After the war Wyndham returned to writing, inspired by the success of his brother who had had four novels published.

After the war ended Sartre established Les Temps Modernes ( Modern Times ), a quarterly literary and political review, and started writing full-time as well as continuing his political activism.