Agarose, the predominant component of agar, is a linear polymer, made up of the repeating monomeric unit of agarobiose.

Agarose is composed of long unbranched chains of uncharged carbohydrate without cross links resulting in a gel with large pores allowing for the separation of macromolecules and macromolecular complexes.

Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change.

Agarose gel electrophoresis is widely used to resolve circular DNA with different supercoiling topology, and to resolve fragments that differ due to DNA synthesis ( Fangman work ).

Agarose as 1 % gel slabs of about 1 mm thickness buffered at high pH ( around 8. 6 ) is traditionally preferred for the electrophoresis as well as the reaction with antibodies.

Agarose has been used widely for immunodiffusion and immunoelectrophoresis, as the agarose fibers functions as an anchor for immunocomplexes.

Agarose gel electrophoresis can be used for the separation of DNA fragments ranging from 50 base pair to several megabases ( millions of bases ) using specialized apparatus.

Agarose gels do not have a uniform pore size, but are optimal for electrophoresis of proteins that are larger than 200 kDa.

* Agarose gel electrophoresis

* Gel electrophoresis, Agarose gel electrophoresis, 2-D electrophoresis, SDS-PAGE

* Agarose gel electrophoresis

* Agarose gel electrophoresis

# REDIRECT Gel electrophoresis # Agarose

So the decision to saturate any type of support depends on the amount of protein required, as described above in the Agarose section of this page.

In the first instance, `` mimesis '' is here used to mean the recalling of experience in terms of vivid images rather than in terms of abstract ideas or conventional designations.

A dominant motive is the poet's longing for his homeland and its boyhood associations: `` Not men-folk, but the fields where I would stray, The stones where as a child I used to play ''.

So in these pages the term `` technology '' is used to include any and all means which could amplify, project, or augment man's control over himself and over other men.

But what a super-Herculean task it is to winnow anything of value from the mud-beplastered arguments used so freely, particularly since such common use is made of cliches and stereotypes, in themselves declarations of intellectual bankruptcy.

This text from Dr. Huxley is sometimes used by enthusiasts to indicate that they have the permission of the scientists to press the case for a wonderful unfoldment of psychic powers in human beings.

The men who speculate on these institutions have, for the most part, come to at least one common conclusion: that many of the great enterprises and associations around which our democracy is formed are in themselves autocratic in nature, and possessed of power which can be used to frustrate the citizen who is trying to assert his individuality in the modern world ''.

Properly used, the present book is an excellent instrument of enlightenment.

This prospect did not please Mrs. King any more than did the possibility that her daughter might marry a Bohemian, but she used it to suggest to Thompson that, `` It is not in her nature to love you ''.

On the other hand, the consensus of opinion is that, used with caution and in conjunction with other types of evidence, the native sources still provide a valid rough outline for the English settlement of southern Britain.

The trouble with this machinery is that it is not used and the reason that it is not used is the absence of a conscious sense of community among the free nations.

In addition to his experiments in reading poetry to jazz, Patchen is beginning to use the figure of the modern jazz musician as a myth hero in the same way he used the figure of the private detective a decade ago.

When different colors are used, she is just as likely to color trees purple, hair green, etc..

Berlin is merely being used by Moscow as a stalking horse.

The collection of information is meaningless unless it is understood and used for a definite purpose.

This is used as a reference for comparing the ohmic heating and the electrical energy obtained from the measured current through the element and the measured voltage across the element.

Your suggested solution, it seems to me, is grossly oversimplified and is inconsistent with your generally realistic attitude toward, and endorsement of, sound planning.

It would challenge sharply not the cult of the motor car itself but some of its ancillary beliefs and practices -- for instance, the doctrine that the fulfillment of life consists in proceeding from hither to yon, not for any advantage to be gained by arrival but merely to avoid the cardinal sin of stasis, or, as it is generally termed, staying put.

It is generally conceded that the Formosan air force is the best by far in Asia, and the army the best trained.

The total adverse impact of disease, insects, fire, weather, destructive animals, and other forces on the uses and values of forest resources is not generally recognized.

Any alteration of one of these factors is distortion, although we generally use that word only for effects so pronounced that they can be stated quantitatively on the basis of standard tests.

Now, the machine has been improved to a point where it is generally more economical than oil heat at temperatures down to 15 degrees.

It is generally an inaccurate method of rating, for the horsepower is that of the compressor motor, and many other components beside it determine how much cooling you'll get.

Above this point there is no generally used parallel ladder.

Advances in equipment and fabrication techniques give the sign or display manufacturer an extremely wide choice of production techniques, ranging from injection molding for intricate, smaller-size, mass-production signs ( generally 5000 units is the minimum ) to vacuum and pressure forming for larger signs of limited runs.

Brown ( 1959 ) has reviewed generally the various methods of assaying TSH, and the reader is referred to her paper for further information on the subject.

The great majority of present-day linguists fall into one or more of a number of overlapping types: those who are convinced that tone cannot be analysed, those who are personally scared of tone and tone languages generally, those who are convinced that tone is merely an unnecessary marginal feature in those languages where it occurs, those who have no idea how to proceed with tone analysis, those who take a simplistic view of the whole matter.

My answer is in the negative because I believe that total capital demands during the Sixties will continue to press against available supplies, and interest rates will generally tend to be firm at high levels.

In general, it appears that trustees and board members attempt to represent the public interest in their administration of educational policy, and this is made easier by the fact that the dominant values of the society are middle-class values, which are generally thought to be valid for the entire society.

In his analysis, however, he touches upon but fails to explore an idea, generally neglected in discussions of the book, which I believe is central to its art -- the importance of human hands as a recurring feature of the narrative.

The respectability which money confers implies a different etiquette, and, upon taking up the life of a London gentleman, Pip must learn from Herbert Pocket that `` the spoon is not generally used over-hand, but under ''.

Because of undesirable flavors, odors, colors, and generally low palatability associated with radiation treatment of this magnitude, the inactivation of enzymes is best accomplished prior to irradiation by the conventional heat-processing methods of blanching.

The polyester urethane foam is generally produced with adipic acid polyesters ; ;

An electrostatic system suffers generally from image plane curvature leading to defocusing in the peripheral image region if a flat viewing screen ( or interstage coupler ) is utilized, while a magnetic system requires accurate adjustment of the solenoid, which is heavy and bulky.

The packing efficiency, F.R., of fiber plates did not receive much attention in the literature, probably as it is high for the larger fibers generally used, until rather recently.

Intermarriage, which is generally regarded as a threat to Jewish survival, was regarded not with horror or apprehension but with a kind of mild, clinical disapproval.

Beer, generally fermented from barley, is an old alcoholic beverage.