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If the DNA is to be used after separation on the agarose gel, it is best to avoid exposure to UV light by using a blue light excitation source such as the XcitaBlue UV to blue light conversion screen from Bio-Rad or Dark Reader from Clare Chemicals.
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If and DNA
If the F-plasmid that is transferred has previously been integrated into the donor ’ s genome some of the donor ’ s chromosomal DNA may also be transferred with the plasmid DNA.
If the cell is synthesizing DNA each pore complex needs to transport about 100 histone molecules per minute.
If the molecules to be separated contain radioactivity, for example in DNA sequencing gel, an autoradiogram can be recorded of the gel.
If the DNA sequence at a particular locus varies between individuals, the different forms of this sequence are called alleles.
If DNA was the genetic material, then DNA from the labeled phage would have to enter the bacterial cells, and carry the radioactive phosphorus with it.
If researchers were trying to initially determine the chromosomal location of a particular disease gene, they would analyze the DNA of members of a family afflicted by the disease, and look for RFLP alleles that show a similar pattern of inheritance as that of the disease ( see Genetic linkage ).
If the gene encoding the protein can be identified it is currently much easier to sequence the DNA and infer the protein sequence.
# If some of the DNA fragments are larger than 15 kb, then prior to blotting, the gel may be treated with an acid, such as dilute HCl, which depurinates the DNA fragments, breaking the DNA into smaller pieces, thus allowing more efficient transfer from the gel to membrane.
# If alkaline transfer methods are used, the DNA gel is placed into an alkaline solution ( typically containing sodium hydroxide ) to denature the double-stranded DNA.
For example, in a negative feedback loop, the transcription factor acts as its own repressor: If the transcription factor protein binds the DNA of its own gene, it will down-regulate the production of more of itself.
If positive clones can be obtained from this procedure, the DNA is sequenced and PCR primers are chosen from sequences flanking such regions to determine a specific locus.
If the DNA was resolved by gel electrophoresis, the DNA can be visualized either by silver staining ( low sensitivity, safe, inexpensive ), or an intercalating dye such as ethidium bromide ( fairly sensitive, moderate health risks, inexpensive ), or as most modern forensics labs use, fluorescent dyes ( highly sensitive, safe, expensive ).
If that is the position, the decision you have to reach, on all the evidence, is whether you are sure that it was the Defendant who left that stain or whether it is possible that it was one of that other small group of men who share the same DNA characteristics.
If this enzyme reaches a " hemimethylated " portion of DNA ( where methylcytosine is in only one of the two DNA strands ) the enzyme will methylate the other half.
If and is
If his dancers are sometimes made to look as if they might be creatures from Mars, this is consistent with his intention of placing them in the orbit of another world, a world in which they are freed of their pedestrian identities.
If a work is divided into several large segments, a last-minute drawing of random numbers may determine the order of the segments for any particular performance.
If Wilhelm Reich is the Moses who has led them out of the Egypt of sexual slavery, Dylan Thomas is the poet who offers them the Dionysian dialectic of justification for their indulgence in liquor, marijuana, sex, and jazz.
If he is the child of nothingness, if he is the predestined victim of an age of atomic wars, then he will consult only his own organic needs and go beyond good and evil.
If he thus achieves a lyrical, dreamlike, drugged intensity, he pays the price for his indulgence by producing work -- Allen Ginsberg's `` Howl '' is a striking example of this tendency -- that is disoriented, Dionysian but without depth and without Apollonian control.
If love reflects the nature of man, as Ortega Y Gasset believes, if the person in love betrays decisively what he is by his behavior in love, then the writers of the beat generation are creating a new literary genre.
If the existent form is to be retained new factors that reinforce it must be introduced into the situation.
If we remove ourselves for a moment from our time and our infatuation with mental disease, isn't there something absurd about a hero in a novel who is defeated by his infantile neurosis??
If many of the characters in contemporary novels appear to be the bloodless relations of characters in a case history it is because the novelist is often forgetful today that those things that we call character manifest themselves in surface behavior, that the ego is still the executive agency of personality, and that all we know of personality must be discerned through the ego.
If our sincerity is granted, and it is granted, the discrepancy can only be explained by the fact that we have come to believe hearsay and legend about ourselves in preference to an understanding gained by earnest self-examination.
If and be
If she, Pamela, were being held responsible for his crimes, then hers must be the final act of expiation.
If he showed signs of collecting his rifle and going back with his deputy to the ranch he would be shot down instantly.
If communications work, his decision would be instantly known in all command posts that would originate the actual go order.
If the Union conceded this to them, the same right must be conceded to each remaining state whenever it saw fit to secede: This would destroy the federal balance between it and the states, and in the end sacrifice to the sovereignty of the states all the liberty the citizens had gained by their Union.
If an automobile were approaching him, he would know what was required of him, even though he might not be able to act quickly enough.
If I now risk some comparisons with Sons And Lovers let it be clear that I am not comparing the two works or judging their merits ; ;
If in any one calculation Ptolemy had had to invoke 83 epicycles all at once, while Copernicus never required more than one third this number, then ( in the sense obvious to Margenau ) Ptolemaic astronomy would be simpler than Copernican.
If `` Jack the Courtier '' is really to be taken as Swift, the following remark is obviously Steele's comment on Swift's change of parties and its effect on their friendship: `` I assure you, dear Jack, when I first found out such an Allay in you, as makes you of so malleable a Constitution, that you may be worked into any Form an Artificer pleases, I foresaw I should not enjoy your Favour much longer ''.
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