In addition to other treatments, treating the sections with normal sheep serum for half an hour before layering Af did not reduce nonspecific staining.

Just before game time, Robinson's pretty wife, Connie informed him that an addition to the family can be expected late next summer.

File: Admiralty office Whitehall 1760 D Cunego. jpg | Old Admiralty ( Ripley Building ) in 1760 before addition of the Adam screen

In addition, the seed germination period can be 50 days or more, which increases the risk of the seeds rotting before they germinate.

In addition, Augustus wanted to avenge the defeat of C. Antonius at Histria ( Sinoe ) 32 years before and to recover the lost standards.

In addition to their earlier appearance together on Courtship, Benet guest-starred with him on his The Magician series in 1973, did an episode of The Love Boat with him in 1977, and guested on his The Incredible Hulk program in 1980 just before they divorced.

In addition, works published before 1964 that did not have their copyrights renewed 28 years after first publication year also are in the public domain, except that books originally published outside the US by non-Americans are exempt from this requirement, if they are still under copyright in their home country ( see How Can I Tell Whether a Copyright Was Renewed for more details ).

In the early 20th century, before enzymology was well understood, colloids were thought to be the key to the operation of enzymes ; i. e., the addition of small quantities of an enzyme to a quantity of water would, in some fashion yet to be specified, subtly alter the properties of the water so that it would break down the enzyme's specific substrate, such as a solution of ATPase breaking down ATP.

In addition, the number of motor cars on the roads rose from 3 million to 5 million from 1945 to 1951, and seaside holidays were taken by far more people than ever before.

This led to the addition of the number sign (#, sometimes called ' octothorpe ,' ' pound ' or ' diamond ' in this context-' hash ' or ' gate ' in the UK ) and asterisk or " star " (*) keys as well as a group of keys for menu selection: A, B, C and D. In the end, the lettered keys were dropped from most phones, and it was many years before these keys became widely used for vertical service codes such as * 67 in the United States of America and Canada to suppress caller ID.

In addition, his retirement cottage outside of Plattsburg, MO burned to the ground before his death in 1886.

City walls are a minor addition to Cities and Knights that increase the number of resource and commodity cards a player is allowed in their hand before having to discard on a roll of 7.

In addition to performing for the Pope, the singer participated in a live broadcast on British television for Christmas Eve in 1997, before she flew home to County Donegal to join her family at midnight Mass.

In addition, speakers of colloquial English frequently contract forms of to be after pronouns or before the word not.

In addition, the EPIC concept depends on compiler capabilities that had never been implemented before, so more research was needed.

In addition to the mandates, the League itself governed the Territory of the Saar Basin for 15 years, before it was returned to Germany following a plebiscite, and the free city of Danzig ( now Gdańsk, Poland ) from 15 November 1920 to 1 September 1939.

In addition the server may send " Unsolicited Notifications " that are not responses to any request, e. g. before it times out a connection.

The Lincos " dictionary ", intended to be transmitted first before any additional messages, begins with a simple pattern of pulses intended to establish the terminology for natural numbers and basic arithmetic ( addition, subtraction, multiplication and division ) in base two.

In addition, the Soviets provided Germany with access to the Northern Sea Route for both cargo ships and raiders ( though only the commerce raider used the route before the German invasion ), which forced Britain to protect sea lanes in both the Atlantic and the Pacific.

In addition, these " conditional " ordinances on behalf of the dead are performed only when a deceased person's genealogical information has been submitted to a temple and correctly processed there before the ordinance ritual is performed.

Nintendo of America also had guidelines before 1993 that had to be followed by its licensees to make games for the Nintendo Entertainment System, in addition to the above content guidelines:.

For added security, secret numbers could be combined with ( usually modular addition ) each code group before transmission, with the secret numbers being changed periodically ( this was called superencryption ).

In addition, PTs work with individuals to prevent the loss of mobility before it occurs by developing fitness and wellness-oriented programs for healthier and more active lifestyles, providing services to individuals and populations to develop, maintain and restore maximum movement and functional ability throughout the lifespan.

In addition to performance footage, the film features interviews from musicians such as the Germs frontman Darby Crash, who committed suicide shortly before the debut of the film.

Although the whole family Poeciliidae is known as " live bearers ", there are egg-scattering species with external fertilization in addition to the true live-bearing viviparous and ovoviviparous species, which have internal fertilization and the eggs hatch before being laid, so the female gives birth to live young.

In San Francisco he has worked with Brew Moore, Charlie Mingus, and other `` swinging '' musicians of secure reputation, thus placing himself within established jazz traditions, in addition to being a part of the San Francisco `` School ''.

And this in addition to his usual fear of being among people of high society, his fear of making some inane or inappropriate remark.

Thus, there has come into being a situation in which the state must raise all of its own revenues and, in addition, must give assistance to its local governments.

But Schnabel was a great teacher in addition to being a great performer, and the fact that four of the ten versions I listened to are by Schnabel pupils ( Clifford Curzon, Frank Glazer, Adrian Aeschbacher, and Victor Babin ) also sheds light on the master's pedagogical skills.

In addition, the effects of the 1988 earthquake, which killed more than 25, 000 people and made 500, 000 homeless, are still being felt.

The high price was likely due to the rare documents and packaging offered in the sale in addition to the computer, including the original packaging ( with the return label showing Steve Jobs ' parents ' address, the original Apple Computer Inc ' headquarters ' being their garage ), a personally typed and signed letter from Jobs ( answering technical questions about the computer ), and the original invoice showing ' Steven ' as the salesman.

In addition to being married to Jochebed, Amram is also described in the Bible as having been related to Jochebed prior to the marriage, although the exact relationship is uncertain ; some Greek and Latin manuscripts of the Septuagint state that Jochebed was Amram's father's cousin, and others state that Amram was Jochebed's cousin, but the Masoretic text states that he was Jochebed's nephew.

As a public hygienic measure in addition to regular cleaning, antimicrobial copper alloys are being installed in healthcare facilities and in a subway transit system.

Titration involves the addition of a reactant to a solution being analyzed until some equivalence point is reached.

The latter flame, in addition, offers a more reducing environment, being ideally suited for analytes with high affinity to oxygen. A laboratory flame photometer that uses a propane operated flame atomizer

Amputations are traumatic experiences that reduce the quality of life for patients in addition to being expensive.

The prehistory of arithmetic is limited to a small number of artifacts which may indicate conception of addition and subtraction, the best-known being the Ishango bone from central Africa, dating from somewhere between 20, 000 and 18, 000 BC although its interpretation is disputed.

In addition to this, there are certain circumstances in which some candidates win what are known as overhang seats when the seats are being distributed.

Most lipids have some polar character in addition to being largely nonpolar.

In addition, if all human hemoglobin were free in the plasma rather than being contained in RBCs, the circulatory fluid would be too viscous for the cardiovascular system to function effectively.

In addition to being buried with her wand, she had received great riches which included horses, a wagon and an Arabian bronze pitcher.

The pope, in addition to being the Bishop of Rome and spiritual head of the Catholic Church, is also the Patriarch of the Latin Rite.

Several scholars consider the book to be anonymous, with verse 1: 1 being a later addition.

In addition to English being the film's primary language, French, Latin, and Scottish Gaelic are also spoken.

Today, there is a palette of attack techniques that a block cipher must be secure against, in addition to being robust against brute force attacks.

In 1846 Robert Smirke was replaced as the Museum's architect by his brother Sydney Smirke, whose major addition was the Round Reading Room 1854 – 1857 ; at in diameter it was then the second widest dome in the world, the Pantheon in Rome being slightly wider.

The next major addition was the White Wing 1882 – 1884 added behind the eastern end of the South Front, the architect being Sir John Taylor.

In 1604 James I ordered some further changes, the most significant of these being the addition to the Catechism of a section on the Sacraments.

In addition to being a place of worship, the cathedral or parish church was used by the community in other ways.

And being a citizen often meant being subject to the city's law in addition to having power in some instances to help choose officials.