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Protein sequence can be determined by Edman degradation, in which the N-terminal residues are hydrolyzed from the chain one at a time, derivatized, and then identified.
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Protein and sequence
* Protein sequencer, a machine used to automatically produce a sequence readout from a biological protein sample
:* E Complex-U1 binds to the GU sequence at the 5 ' splice site, along with accessory proteins / enzymes ASF / SF2, U2AF ( binds at the Py-AG site ), SF1 / BBP ( BBP = Branch Binding Protein );
Most such methods do rely on comparisons between the sequence to be predicted and sequences of known structure in the Protein Data Bank and thus account for environment indirectly, assuming the target and template sequences share similar cellular contexts.
Protein methylation typically takes place on arginine or lysine amino acid residues in the protein sequence.
Protein C is activated in a sequence that starts with Protein C and thrombin binding to a cell surface protein thrombomodulin.
Protein Data Bank ( PDB ): repository for protein sequence and structural information
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its secondary, tertiary, and quaternary structure from its primary structure.
A domain that covers an entire sequence is called the homeomorphic domain by PIR ( Protein Information Resource ).
; Protein threading: scans the amino acid sequence of an unknown structure against a database of solved structures.
* Protein: sequence database
Protein sequencing is a technique to determine the amino acid sequence of a protein, as well as which conformation the protein adopts and the extent to which it is complexed with any non-peptide molecules.
It was established by The Scripps Research Institute to predict protein structure from protein sequence in the context of the 6th biannual CASP, or Critical Assessment of Techniques for Protein Structure Prediction.
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of a protein's tertiary structure from its primary structure.
The Ramachandran plot utilizes mathematical equations to determine the possible angles of the amino acids within the primary sequence of Protein Z.
PIR, hosted by the National Biomedical Research Foundation ( NBRF ) at the Georgetown University Medical Center in Washington, DC, USA, is heir to the oldest protein sequence database, Margaret Dayhoff's Atlas of Protein Sequence and Structure, first published in 1965.
* Protein sequence alignment ( sequence comparison, incl.
Protein and can
Protein, though used colloquially to refer to any polypeptide, refers to larger or fully functional forms and can consist of several polypeptide chains as well as single chains.
Protein microarrays and high throughput ( HT ) mass spectrometry ( MS ) can provide a snapshot of the proteins present in a biological sample.
Protein Phosphatase-1 can take out these phosphate moieties and make glycogen synthase active.
) The precise structure of the prion is not known, though they can be formed by combining PrP < sup > C </ sup >, polyadenylic acid, and lipids in a Protein Misfolding Cyclic Amplification ( PMCA ) reaction.
Solved structures are usually deposited in the Protein Data Bank ( PDB ), a freely available resource from which structural data about thousands of proteins can be obtained in the form of Cartesian coordinates for each atom in the protein.
Protein binding can be extraordinarily tight and specific ; for example, the ribonuclease inhibitor protein binds to human angiogenin with a sub-femtomolar dissociation constant (< 10 < sup >− 15 </ sup > M ) but does not bind at all to its amphibian homolog onconase (> 1 M ).
For example, synthetic peptides can be used as probes to see where protein-peptide interactions occur-see the page on Protein tags.
* Protein domain, a part of a protein that can exist independently of the rest of the protein chain
Protein kinases can become mutated, stuck in the " on " position, and cause unregulated growth of the cell, which is a necessary step for the development of cancer.
It can generate pairwise or multiple alignments and identify a query sequence's structural neighbors in the Protein Data Bank ( PDB ).
The CATH database can be accessed at CATH Protein Structure Classification.
* Iron Regulatory Protein are proteins that can bind to iron response elements and affect mRNA translation, thereby regulating the synthesis of specific proteins.
Protein structural modeling can be performed by examining how well the amino acid substitutions fit into the core of the three-dimensional structure.
In addition to possibly affecting the product, low pH can cause Protein A / G itself to leak off the column and appear in the eluted sample.
Protein misfolding can result in a variety of diseases including Alzheimer's disease, cancer, Creutzfeldt – Jakob disease, cystic fibrosis, Huntington's disease, sickle-cell anemia, and type II diabetes.
HOP ( the Hsp70 / Hsp90 Organizing Protein ) can bind to both Hsp70 and Hsp90 at the same time, and mediates the transfer of peptides from Hsp70 to Hsp90.
SPACEHAB middeck experiments that were activated included Immune-1, which will look at the immune systems of rats in orbit, and the Commercial Protein Crystal Growth package, which is attempting to grow large, well-ordered protein crystals so that their structures can be more easily studied.
Protein complex formation sometimes serves to activate or inhibit one or more of the complex members and in this way, protein complex formation can be similar to phosphorylation.
* Protein levels can be analysed by Mass spectrometry, which can be compared only to QPCR data, as microarray data is relative and not absolute.
Protein electrophoresis and immunoglobulin levels can show increased IgA in 50 % of all patients.
Protein sequences can provide data about the biological function and evolution of proteins and protein domains.
Protein structures range in size from tens to several thousand residues Very large aggregates can be formed from protein subunits: for example, many thousand actin molecules assemble into a microfilament.
Protein structures can be grouped based on their similarity or a common evolutionary origin.
Protein and be
In the description of protein structure, in particular in the Protein Data Bank file format, a heteroatom record ( HETATM ) describes an atom as belonging to a small molecule cofactor rather than to be part of a biopolymer chain.
Factor V Leiden is an autosomal dominant condition that exhibits incomplete dominance and results in a factor V variant that cannot be as easily degraded by aPC ( activated Protein C ).
Protein combining has lost favor as theory ( with even its original proponent, Frances Moore Lappé, rejecting the need for protein combining in 1981 )-a variety of protein sources is considered healthy, but these do not have to be consumed at the same meal.
C-peptide should not be confused with c-reactive protein or Protein C.
In the Protein Crystal Growth experiment, two of the 11 proteins processed – including an enzyme believed to be key to the replication of AIDS – did not produce crystals suitable for analysis.
Protein precipitated will be large enough to be visible.
Protein conversion uses techniques to convert inedible parts of the animal, such as keratin in feathers and skin, into digestible protein to be used for livestock and pet food.
N-terminal residue sequencing resulting in five to ten amino acid may be sufficient to identify a Protein of Interest ( POI ).
Protein interactions are often too weak or transient to be easily detected, but, by crosslinking, the interactions can be captured and analyzed.
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