# The nuclear envelope becomes discontinuous and the DNA inside it is fragmented in a process referred to as karyorrhexis.

# DNA wraps around histone proteins forming nucleosomes ; the " beads on a string " structure ( euchromatin ).

# Higher-level DNA packaging of the 30 nm fibre into the metaphase chromosome ( during mitosis and meiosis ).

# Simple and concise definition: Chromatin is DNA plus the proteins ( and RNA ) that package DNA within the cell nucleus.

# A biochemists ’ operational definition: Chromatin is the DNA / protein / RNA complex extracted from eukaryotic lysed interphase nuclei.

# The DNA + histone

# A eukaryotic cell transcribes the DNA ( from genes ) into RNA ( pre-mRNA ).

# A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA ( Reverse transcriptase requires this double-stranded segment as a primer to start its operation.

# To synthesize an additional DNA strand, you need to digest the RNA of the hybrid strand, using an enzyme like RNase H.

# After digestion of the RNA, a single stranded DNA ( ssDNA ) is left and because single stranded nucleic acids are hydrophobic, it tends to loop around itself.

# From the hairpin loop, a DNA polymerase can then use it as a primer to transcribe a complementary sequence for the ss cDNA.

# REDIRECT DNA

# REDIRECT DNA microarray

# The DNA passes through a separate sugar transport protein ( ptsG ) in the inner membrane, and immediately circularises using the cos sites, 12-base G-C rich cohesive " sticky ends ".

# Host DNA gyrase puts negative supercoils in the circular chromosome, causing A-T rich regions to unwind and drive transcription.

# The host cell, containing a dormant phage genome, experiences DNA damage due to a high stress environment, and starts to undergo the SOS response.

# RecA ( a cellular protein ) detects DNA damage and becomes activated.

# Normally RecA * binds LexA ( a transcription repressor ), activating LexA auto-protease activity, which destroys LexA repressor allowing production of DNA repair proteins.

# Cleaved cI can no longer dimerise, and loses its affinity for DNA binding.

# The phage genome is still inserted in the host genome and needs excision for DNA replication to occur.

# C-terminal involved in downregulation of DNA binding of the central domain: residues 356-393.

# Complementary DNA then binds to the U5 ( non-coding region ) and R region ( a direct repeat found at both ends of the RNA molecule ) of the viral RNA

# The primer then ‘ jumps ’ to the 3 ’ end of the viral genome and the newly synthesised DNA strands hybridizes to the complementary R region on the RNA

# The first strand of complementary DNA ( cDNA ) is extended and the majority of viral RNA is degraded by RNAse H

# As exploratory data analysis, an ANOVA is an organization of an additive data decomposition, and its sums of squares indicate the variance of each component of the decomposition ( or, equivalently, each set of terms of a linear model ).

# Closely related to the ANOVA is a linear model fit with coefficient estimates and standard errors.

# The accused person ( defendant ) is addressed by name ;

# The charge against the accused person is read, including the alleged date, time, and place of offense ; and,

# The accused person is asked formally how he or she pleads.

# Assume the first item is largest.

# Look at each of the remaining items in the list and if it is larger than the largest item so far, make a note of it.

# The last noted item is the largest in the list when the process is complete.

# The Lambeth Conference ( first held in 1867 ) is the oldest international consultation.

# The Primates ' Meeting ( first met in 1979 ) is the most recent manifestation of international consultation and deliberation, having been first convened by Archbishop Donald Coggan as a forum for " leisurely thought, prayer and deep consultation ".

# Deontological ethics, notions based on ' rules ' i. e. that there is an obligation to perform the ' right ' action, regardless of actual consequences ( epitomized by Kant's notion of the Categorical Imperative )

# A Murder is Announced ( 1950 )

# Civil aviation, held by Armavia national airline ( owned by Mikhail Baghdasarov, who is thought to be close to President Serge Sarkisian )

# There is always an integer number of electrons orbiting the nucleus.

# The Slater-type orbital ( STO ) is a form without radial nodes but decays from the nucleus as does the hydrogen-like orbital.

# A person who is skilled at some activity.

# unaided by the Holy Spirit, no person is able to respond to God ’ s will ;

# grace is resistible ; and

# Prior to being drawn and enabled, one is unable to believe … able only to resist.

# Having been drawn and enabled, but prior to regeneration, one is able to believe … able also to resist.

# After one believes, God then regenerates ; one is able to continue believing … able also to resist.

# Upon resisting to the point of unbelief, one is unable again to believe … able only to resist.

# The carbon-hydrogen ratio is high.

# It is easily harvested.