Although the newborn ibex died shortly after birth due to physical defects in its lungs it is the first time an extinct animal has been cloned, and may open doors for saving endangered and newly extinct species by resurrecting them from frozen tissue.

The fresh tissue or cell suspension is frozen rapidly ( cryofixation ), then fractured by simply breaking or by using a microtome while maintained at liquid nitrogen temperature.

Embedding can also be accomplished using frozen, non-fixed tissue in a water-based medium.

In the last couple of decades, developments in electron microscopy, immunofluorescence, and the use of frozen tissue sections have enhanced the detail that can be observed in tissues.

In Sep 2004 Prof Donnez of Louvain in Belgium reported the first successful ovarian birth from frozen ovarian tissue.

They are also very useful in immunohistochemistry, which detect antigen in fixed tissue sections and immunofluorescence test, which detect the substance in a frozen tissue section or in live cells.

Or one should simply process the tissue utilizing a method approximating the Mohs method ( described in most basic histopathology text books or described in this reference ) during frozen section processing.

The tissue is then prepared for viewing under a microscope using either chemical fixation or frozen section.

In this method, the tissue is frozen and sliced thinly using a microtome mounted in a below-freezing refrigeration device called the cryostat.

With Cryoablation, a supercooled catheter is used ( cooled by nitrous oxide gas ), and the tissue is frozen to-10 ° C.

Fixation is done either by the fixed tissue method with paraffin, or by frozen section.

With frozen tissue sectioning, the tissue is immediately frozen prior to processing ( frozen section ).

The frozen tissue block embedded in a frozen cutting medium, or the paraffin fixed tissue is cut using a very fine knife called a microtome.

A cryostat is a micotome mounted inside a freezer for processing frozen tissue.

the passage and rhythm of time changed, and when I remember back to what happened then, each event is a separate and frozen incident.

A refrigerated item could also be heated and served in less time than is required for frozen foods of the same type.

It is frozen from October to late May and has a maximum flow in June with the snowmelt.

For nine months of the year the ground is covered with snow, and the frozen rivers become navigable roads.

Every year, on the last Sunday in April, there is an ice fishing competition in the frozen estuarine waters of the Anadyr River's mouth.

While amber is not actually named, it is called the concreti maris purgamentum, " the leavings of the frozen sea " after the spring melt.

It is known that since 1720, the Baltic Sea has frozen over entirely only 20 times.

In his article The Old Man and the Daiquiri, Wayne Curtis tells us how Hemingway ’ s “ home bar also held a bottle of Bacardí rum .” Hemingway wrote in Islands in the Stream “… this frozen daiquirí, so well beaten as it is, looks like the sea where the wave falls away from the bow of a ship when she is doing thirty knots .”

The Croatian parliament has passed a law that no bus should be older than 12 years-however, this decision is currently frozen because of the high cost for bus operators.

Boudin is usually made daily as it doesn't keep well for very long, even when frozen.

The North American Snowmobile Festival ( NASF ) is held on frozen Lake Cadillac every winter.

In the Halo franchise, the weak, low-ranking Grunts of the Covenant originate on a frozen exoplanet named Balaho, where methane is a primary constituent of the atmosphere, prevents the planet becoming even more frigid than it already is due to its distance from its parent star, and thus, Grunts have evolved to utilize the gas for respiration.

Most foods can be preserved in soil that is very dry and salty ( thus a desiccant ), or soil that is frozen.

Belgian journalist Jo Gérard has claimed that a family manuscript dated 1781 recounts that potatoes were deep-fried prior to 1680 in what was then the Spanish Netherlands and is now present-day Belgium, in the Meuse valley: " The inhabitants of Namur, Andenne, and Dinant, had the custom of fishing in the Meuse for small fish and frying, especially among the poor, but when the river was frozen and fishing became hazardous, they cut potatoes in the form of small fish and put them in a fryer like those here ".

The J. R. Simplot Company is credited with successfully commercializing French fries in frozen form during the 1940s.

It is estimated that 80 % of households in the UK buy frozen fries each year.

Canada's McCain Foods is the world's leading producer of frozen fries.

The argument is, the schools have been more or less frozen for centuries, and reflect a culture that simply no longer exists.

For example, air hoar is a deposit of hoar frost on objects above the surface, such as tree branches, plant stems, wires ; surface hoar is formed by fernlike ice crystals directly deposited on snow, ice or already frozen surfaces ; crevasse hoar consists of crystals that form in glacial crevasses where water vapour can accumulate under calm weather conditions ; depth hoar refers to cup shaped, faceted crystals formed within dry snow, beneath the surface.

In fact the product was sliced, pre-fried, sometimes had dextrose added, was then frozen, shipped, and re-fried and then had salt added.

They may also be frozen whole or sliced.

Cars and game objects can realistically be sliced into pieces, flattened, frozen, shattered, shredded, impaled, lit on fire ( and subsequently burn to a crisp and fall into ashes ), and more.

In experimental work at University of Alaska Fairbanks, a 1000-litre digested using psychotropics harvested from " mud from a frozen lake in Alaska " has produced 200 – 300 liters of methane per day, about 20 – 30 % of the output from dig esters in warmer climates.

In 1916, Khabarovsk Bridge across the Amur was completed, allowing Trans-Siberian trains to cross the river without using ferries ( or temporary rail tracks over the frozen river in winter ).

In the dead of winter, the frozen Lena makes for a passable highway for ice truckers using its channel to deliver provisions to far-flung outposts.

Bodies were preserved using mummification techniques and were also naturally frozen in solid ice from water seeping into the tombs.

Since then machines that freeze biological samples using programmable steps, or controlled rates, have been used all over the world for human, animal and cell biology – ' freezing down ' a sample to better preserve it for eventual thawing, before it is deep frozen, or cryopreserved, in liquid nitrogen.

Dr Christopher Chen, Australia, reported the world ’ s first pregnancy using slow frozen oocytes from a British Controlled Rate freezer in 1986.

In T: 2, this Terminator was even frozen using liquid nitrogen and shattered, but the pieces simply flowed back together after thawing.

* How to deal with a frozen air-supply system using a redundant back-up system.

Cutting frozen tuna using a band saw in the Tsukiji fish market in Tokyo, Japan ( 2002 )

Another way to create a frozen daiquiri ( mostly fruit-flavored variants ) is by using frozen limeade, providing the required texture, sweetness and sourness all at once.

Swanson stopped using the name " TV Dinner " in 1962 ; in the United States the term remains synonymous with any prepackaged dinner purchased frozen in a supermarket and heated at home.

However, Swanson, a large producer of canned and frozen poultry in Omaha, Nebraska, was able to promote the widespread sales and adaptation of frozen dinner by using its nationally-recognized brand name with an extensive national marketing campaign nicknamed " Operation Smash " and the clever advertising name of " TV Dinner ," which tapped into the public's excitement around the new device.

: Agricultural: Farming and livestock preserved by using frozen embryos.

A hull using 5, 000 kg of hemp fibre Pykrete was frozen in a cold-store, then launched in Portsmouth harbour for a planned trip across the Solent to Cowes.

Rocky uses his intelligence to explain to Diana the sighted words like pillowy, clouds, red and green by using cotton balls as a touchable vision of pillowy clouds, a warm rock to explain red and pink, and a frozen rock to explain icy blue.

The New York Board of Trade was featured in the 1983 movie Trading Places, where two rich brothers came up with a scheme to buy out all of the frozen concentrate orange juice market using inside information from the Department of Agriculture.

In experimental work at University of Alaska Fairbanks, a 1000 litre biogas digester using psychrophiles harvested from " mud from a frozen lake in Alaska " has produced 200 – 300 litres of methane per day, about 20 – 30 % of the output from digesters in warmer climates.

Anderson's method is not in use by the chain today, having changed when the company switched from using fresh beef and fresh onion to small, frozen square patties ( originally supplied by Swift & Company ) which are cooked atop a bed of rehydrated onions laid out on a grill.

In 1980, Häagen-Dazs unsuccessfully sued Frusen Glädjé, an American ice cream maker, whose name without the acute accent is Swedish for " frozen delight ", for using similar foreign branding strategies.