The cell used as the donor for the cloning of Dolly was taken from a mammary gland, and the production of a healthy clone therefore proved that a cell taken from a specific part of the body could recreate a whole individual.

After cloning was successfully demonstrated through the production of Dolly, many other large mammals have been cloned, including horses and bulls.

In 1964, the play enjoyed yet another incarnation when David Merrick, who had produced the 1955 Broadway production, mounted a hugely successful, Tony Award-winning musical version entitled Hello, Dolly !, with a score by Jerry Herman and starring Carol Channing.

Continuing his production work into the 1980s, Perkins also toured with Chris Hillman as a duo which later developed into the Desert Rose Band, before joining Dolly Parton in 1986, touring and recording with her for several years before moving to Nashville.

* Miss Dolly Dollars Broadway production opened at the Knickerbocker Theatre on September 4 and moved to the New Amsterdam Theatre on October 16 for a total run of 112 performances.

Although the production of Dolly was a scientific breakthrough, it was controversial, since it showed that not only could cloned animals be produced for use in farming, but also that it would now be, in principle, possible to clone a human being.

This production starred Andrea McArdle ( the original Annie of the 1977 Broadway musical Annie ), Jeffrey Coon as Frank Butler, John Scherer as Charlie Davenport, Chris Councill as Buffalo Bill, Mary Martello as Dolly Tate, and Arthur Ryan as Sitting Bull.

On 18 June 2008, while appearing in a production of Hello, Dolly!

She moved to New York City in 1969, appearing off-Broadway in Lend an Ear, and for a year as Minnie Fay in the Broadway production of Hello, Dolly !.

" In 1964, Worley was selected to appear as a stand-in on the original Broadway production of Hello, Dolly!

In June 2010, Lewis ' distinctive voice was in fine form as she told The Jazz Joy and Roy syndicated radio show, " I just did a production of ' Hello Dolly ' at the 5th Avenue Theatre in Seattle and it had to be one of the greatest productions that I have ever done, because I got to just do a character, Dolly Levi, and it was just great.

The production of Dolly the Sheep and also Megan and Morag, the two sheep that led to the production of Dolly, demonstrated that viable sheep can be produced by nuclear transfer from a variety of somatic cell types which have been cultured in vitro.

The crew of people involved in this stage of production include the Cinematographer, Production Manager, the Technical Director, the Boom Operator, the Gaffer, the Dolly grip, the Key Grip and the Stunt coordinator.

On 29 June 2012, it was confirmed that Miss Clune will be appearing as Violet Newstead in the first UK tour production of the Broadway musical penned by Dolly Parton, 9 to 5. A photoshoot involving the initial cast took place in July 2012.

She appeared in a wide variety of roles, and starred in such melodramas as The Witch Girl, A Daughter of the Nile, The Active Life of Dolly of the Dailies ( 1914 ), and Under Southern Skies, her first feature-length production.

The company first showed cars of its own design at the 1902 Hamburg Motor Show, and started manufacturing them in 1906, with Opel-Darracq production being discontinued in 1907.

To make matters more complicated, CBS ' production staff found a reverse angle of the Kay catch that was unavailable to the replay booth and it clearly showed the reception made by Kay.

The production showed that musicals could be profitable Off-Broadway in a small-scale, small orchestra format.

In 1831, Berzelius reported the production of the metal, but Henry Enfield Roscoe showed that Berzelius had in fact produced the nitride, vanadium nitride ( VN ).

showed that fibroblasts exposed to mechanical stimulation have differing levels of ECM production than fibroblasts that are not exposed to mechanical stimulation.

A study on capsaicin production in fruits of C. chinense showed that capsaicinoids are produced only in the epidermal cells of the interlocular septa of pungent fruits, that blister formation only occurs as a result of capsaicinoid accumulation, and that pungency and blister formation are controlled by a single locus, Pun1, for which there exist at least two recessive alleles that result in non-pungency of C. chinense fruits.

Data collected by the World Bank showed that food production per capita fell about 12 percent from 1980 to 1987.

The 2002 TV documentary on the making of the film, The Tramp and the Dictator, presented newly discovered footage of the film production ( shot by Chaplin's elder half-brother Sydney ) which showed Chaplin's initial attempts at the film's ending, filmed before the fall of France.

Scenes from early on in the movie showed paintings lent to the production from the Norton Simon art gallery.

During production of Gertie, he showed all the details to a visitor who claimed to be writing an article about animation.

François Truffaut was inspired by Little Fugitives spontaneous production style when he created The 400 Blows ( 1959 ), saying long afterwards: “ Our New Wave would never have come into being if it hadn ’ t been for the young American Morris Engel, who showed us the way to independent production with fine movie .”

The network has run blocks of classic game shows on Saturday nights, and for the first few months of 2006 programmed back-to-back episodes of Match Game in a block billed as That ' 70s Hour ( a pun on That ' 70s Show ), which showed the original production slate before each episode as well as Match Game trivia and brief clips of an interview with host Gene Rayburn produced shortly before his death.

Some of these dialects showed considerable literary production ; the Sravakachar of Devasena ( dated to the 930s ) is now considered to be the first Hindi book.

The war economy pumped massive investments into new factories and funded round-the-clock munitions production, guaranteeing a job to anyone who showed up at the factory gate.

Although Warner Bros. maintained a high rate of production throughout the 1930s, some pictures showed an uneven quality that reflected " not only the difficulty of shifting to a supervisory system but also the consequences of dispersing authority into the creative ranks ".

Additionally, many theories were developed which showed that the free market would produce the socially optimum equilibrium with regard to production of goods and services, such as the fundamental theorems of welfare economics and general equilibrium theory, which helped prove further that government intervention could only result in making society worse off ( see Pareto efficient ).

Naive CD4 + T cells ( those that have not encountered any antigens before ) recovered from these mice 36 hours post-anti-MHC administration showed decreased responsiveness to the antigen pigeon cytochrome C peptide, as determined by Zap-70 phosphorylation, proliferation, and Interleukin-2 production.

Vasiliyev showed his financiers a marketing study stating that 30 % of Moscow's population fit the profile audience that would be willing to pay for the production, due to changing sensibilities and increasing incomes The Russian theatre community had a prejudice against this kind of play.

Both Personnel and his next feature, The Scar ( Blizna ), were works of social realism with large casts: Personel was about technicians working on a stage production, based on his early college experience, and The Scar showed the upheaval of a small town by a poorly-planned industrial project.

showed that activated monocytes, which is the case in sepsis, express high levels of PD-1 and that triggering monocytes-expressed PD-1 by its ligand PD-L1 induces IL-10 production which inhibits CD4 T-cell function.

Although some Western and East Asian investors also showed their interest in RAF, all of them considered this investment too risky as the local market was too small to support large production and the Russian market was virtually closed due to the complicated political relationship of Russia and Latvia.

Garrick showed an enthusiasm for the theatre very early on and he appeared in a school production around this time in the role of Sergeant Kite in George Farquhar's The Recruiting Officer.

For most of the Islamic period the majority of coins only showed lettering, which are often very elegant despite their small size and nature of production.

Studies have been conducted to isolate the genes involved in the substance's production, so that it could be obtained recombinantly.

Mutations in two regulatory genes of white grapes turn off production of anthocyanins which are responsible for the color of purple grapes.

In addition, interferons induce production of hundreds of other proteins — known collectively as interferon-stimulated genes ( ISGs )— that have roles in combating viruses.

Dwarfing genes enable the carbon that is fixed in the plant during photosynthesis to be diverted towards seed production, and they also help prevent the problem of lodging.

The team that went on to discover the VvMYBA1 and VvMYBA2 genes that control grape colour have suggested that a gene involved in anthocyanin production has been deleted in the subepidermis of Malian, and then subepidermal cells invaded the epidermis to produce Shalistin.

# At some point, northern populations experienced positive selection for lighter skin due to the increased production of vitamin D from sunlight and the genes for darker skin disappeared from these populations.

The majority of toolkit genes are components of signaling pathways, and encode for the production of transcription factors, cell adhesion proteins, cell surface receptor proteins, and secreted morphogens, all of these participate in defining the fate of undifferentiated cells, generating spatial and temporal patterns, which in turn form the body plan of the organism.

However, these genes confer only partial resistance to FHB, and many of the initial sources of resistance were not well adapted to most of the grain production regions of the U. S. While some success has been made in transferring FHB resistance from such exotic sources into adapted cultivars, identification and deployment of FHB resistance already present in local native germplasm and cultivars is providing another means to achieve this goal.

This may include the upregulation and / or downregulation of several genes and their transcription factors, resulting in the production of other cytokines, an increase in the number of surface receptors for other molecules, or the suppression of their own effect by feedback inhibition.

Transferred genes enter the plant cell nucleus and effectively transform the plant cells into factories for the production of opines, which the bacteria use as carbon and energy sources.

Using small extracellularly secreted molecules, they are able to adapt their behavior to turn on genes for light production only when they are at high cell densities.

It has been reported that exposure to corn pollen containing genes for Bacillus thuringiensis ( Bt ) production may weaken the bees ' defense against Nosema .< ref name =" sciam ">

When these genes are expressed in prokaryotic cells for the sake of protein production or purification, the RNA produced directly from transcription need not undergo splicing as the transcript contains only exons.

The X chromosomes of humans and other mammals also contain other genes needed for normal sperm production.

As it contains some non-vinifera genes, it is outlawed by the EU authorities for quality wine production, which is an issue of conflict with the English wine industry.

Both the red and the black raspberry species have albino-like pale-yellow natural or horticultural variants resulting from presence of recessive genes that impede production of anthocyanin pigments.

Some other possible features present in cloning vectors are: vir genes for plant transformation, integrase sites for chromosomal insertion, lacZα fragment for α complementation and blue-white selection, and / or reporter genes in frame with and flanking the MCS to facilitate the production of recombinant proteins fused to the Green fluorescent protein ( GFP ) or to the glutathione S-transferase ( see figure ).

By binding to the promoter region at the start of other genes they turn them on, initiating the production of another protein, and so on.

As a result, mutations in ras genes can lead to the production of permanently activated Ras proteins.

These genes are repressed when lactose is not available to the cell, ensuring that the bacterium only invests energy in the production of machinery necessary for uptake and utilization of lactose when lactose is present.

The F plasmid consists of 28 genes which are mostly required for the production of the pilus.

Congenital adrenal hyperplasia ( CAH ) refers to any of several autosomal recessive diseases resulting from mutations of genes for enzymes mediating the biochemical steps of production of cortisol from cholesterol by the adrenal glands ( steroidogenesis ).

Inherited mutations in 3 genes which all result in increased stability of Hypoxia Inducible Factors ( HIFs ), leading to increased erythropoietin production, have been shown to cause erythrocytosis: