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The serum was measured volumetrically and subsequently dialyzed in the cold for at least 24 hr against three to four changes, approximately 750 ml each, of `` starting buffer ''.
from Brown Corpus
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serum and was
In addition one serum was obtained from a donor ( R. E. ) who had been sensitized 6 years previously.
The cells were washed three times with saline, anti-human serum was added, the cells were resuspended, and the mixture was centrifuged at 1000 rpm for 1 min and examined for agglutination.
Blood samples were allowed to clot at room temperature for 3 hr, centrifuged and the serum was removed.
When paper electrophoresis was to be used for preparation, eight strips of a whole serum sample or a chromatographic fraction concentrated by negative pressure dialysis were run/chamber under the conditions described above.
In 2 of 15 experiments on whole serum a region of agglutinin activity with intermediate anionic binding capacity was detected ( Region 3, Fig. 1 ).
There appeared to be no difference in the distribution of anti-A and anti-B activity in group O serum, though in two group O donors ( J. F. and E. M. ) only one type of agglutinin was found in the regions of low anionic binding capacity ( Figs. 1 and 2 ).
when the insoluble fraction was suspended in a volume of saline equal to that of the original serum sample, no complete antibody activity could be detected.
In other words, a meter glucose reading of 39 mg / dL could be properly obtained from a person whose laboratory serum glucose was 53 mg / dL ; even wider variations can occur with " real world " home use.
On February 3, 1925, Gunnar Kaasen was first in the 1925 serum run to Nome to deliver diphtheria serum from Nenana, over 600 miles to Nome.
Though Balto is the more famous of the two, and was the dog to deliver the serum to Nome after running the final 53-mile leg, it was Togo who made the longest run of the relay, guiding his musher Leonhard Seppala on a 91-mile journey that included crossing the deadly Norton Sound to Golovin.
In 1963 the US Supreme Court ruled that confessions produced as a result of ingestion of truth serum was " unconstitutionally coerced ", and therefore inadmissible.
The first Nobel Prize in Physiology or Medicine was awarded in 1901 to the German physiologist Emil Adolf von Behring, for his work on serum therapy and the development of a vaccine against diphtheria.
He won the first Nobel Prize in Physiology or Medicine in 1901 for developing a serum therapy against diphtheria ( this was helped by Kitasato Shibasaburo and worked on with Emile Roux ) and tetanus.
Behring demonstrated that the injection of toxins was able to be transmitted to another animal by injections of a treated animal's blood serum and used as a means of effecting a cure.
Landsteiner described the regular patterns in which reactions occurred when serum was mixed with red blood cells, thus identifying compatible and conflicting combinations between these blood groups.
In 1925, Nome was the destination of the famous " Great Race of Mercy ", where dog sleds played a large part in transporting diphtheria serum through harsh conditions.
Nenana was the starting point for the 1925 serum run to Nome, where diphtheria antitoxin was transported by rail from Anchorage, and continued by dog sled to Nome.
serum and measured
Approximately 60 different biochemical parameters were classified per organ and measured in serum and urine after 5 and 14 weeks of feeding.
The genetic revolution in studies of human evolution started when Vincent Sarich and Allan Wilson measured the strength of immunological cross-reactions of blood serum albumin between pairs of creatures, including humans and African apes ( chimpanzees and gorillas ).
Blood glucose levels discussed in this article are venous plasma or serum levels measured by standard, automated glucose oxidase methods used in medical laboratories.
Methadone and its major metabolite, EDDP, are often measured in urine as part of a drug abuse testing program, in plasma or serum to confirm a diagnosis of poisoning in hospitalized victims, or in whole blood to assist in a forensic investigation of a traffic or other criminal violation or a case of sudden death.
Silver concentrations in whole blood, plasma, serum or urine may be measured to monitor for safety in exposed workers, to confirm the diagnosis in potential poisoning victims or to assist in the forensic investigation in a case of fatal overdosage.
#" Elevated serum prolactin assay, when measured in the appropriate clinical setting at 10 to 20 minutes after a suspected event, is a useful adjunct for the differentiation of generalized tonic-clonic or complex partial seizure from psychogenic nonepileptic seizure among adults and older children ( Level B ).
Inoculation of throat washings taken from people with pleurodynia into the brains of newborn mice revealed that enteroviruses in the Coxsackie B virus group were likely to be the cause of pleurodynia, and those findings were supported by subsequent studies of IgM antibody responses measured in serum from people with pleurodynia.
Plasma, serum or blood concentrations of sertraline and norsertraline, its major active metabolite, may be measured to confirm a diagnosis of poisoning in hospitalized patients or to aid in the medicolegal investigation of fatalities.
In women, serum estradiol is measured in a clinical laboratory and reflects primarily the activity of the ovaries.
The acute part of AoCRF may be reversible, and the goal of treatment, as with AKI, is to return the patient to baseline renal function, typically measured by serum creatinine.
Blood, serum or plasma topiramate concentrations may be measured using immunoassay or chromatographic methods to monitor therapy, confirm a diagnosis of poisoning in hospitalized patients or to assist in a medicolegal death investigation.
A 90 percent reference interval for serum glucose of 26 to 181 mg / dL has been reported for captured mountain goats ( Oreamnos americanus ), where no effects of the pursuit and capture on measured levels were evident.
In addition, serum alpha-fetoprotein ( AFP ) and lactate dehydrogenase ( LDH ) should be measured in young girls and adolescents with suspected ovarian tumors because the younger the patient, the greater the likelihood of a malignant germ cell tumor.
in other words, each 1 g / dL decrease of albumin will decrease 0. 8 mg / dL in measured serum Ca and thus 0. 8 must be added to the measured Calcium to get a corrected Calcium value.
in other words, each 1 g / L decrease of albumin, will decrease 0. 02 mmol / L in measured serum Ca and thus 0. 02 must be added to the measured value to take this into account and get a corrected calcium.
The amount of growth, measured as the diameter of the colony, is roughly proportional to the amount of phenylalanine in the serum.
In the setting of low serum albumin ( frequently seen in patients with chronic diseases, hepatic disease or even long term hospitalization ), the formula for corrected calcium is: Corrected calcium ( mg / dL ) = measured total Ca ( mg / dL ) + 0. 8 ( 4. 0-serum albumin ), where 4. 0 represents the average albumin level in g / dL ; in SI units: Corrected calcium ( mmol / L ) = measured total Ca ( mmol / L ) + 0. 02 ( 40-serum albumin ), where 40 represents the average albumin level in g / L.
Transient increases in alkaline phosphatase related to Neupogen were reported in 21 % of the patients who had serum chemistries measured ; most were mild-to-moderate.
Serum iron levels ( i. e., iron not part of the hemoglobin in red cells ) may be measured directly in the blood, but these levels increase immediately with iron supplementation ( the patient must stop supplements for 24 hours ), and pure blood-serum iron concentration in any case is not as sensitive as a combination of total serum iron, along with a measure of the serum iron-binding protein levels ( TIBC ).