In addition one serum was obtained from a donor ( R. E. ) who had been sensitized 6 years previously.

The cells were washed three times with saline, anti-human serum was added, the cells were resuspended, and the mixture was centrifuged at 1000 rpm for 1 min and examined for agglutination.

Blood samples were allowed to clot at room temperature for 3 hr, centrifuged and the serum was removed.

The serum was measured volumetrically and subsequently dialyzed in the cold for at least 24 hr against three to four changes, approximately 750 ml each, of `` starting buffer ''.

When paper electrophoresis was to be used for preparation, eight strips of a whole serum sample or a chromatographic fraction concentrated by negative pressure dialysis were run/chamber under the conditions described above.

In 2 of 15 experiments on whole serum a region of agglutinin activity with intermediate anionic binding capacity was detected ( Region 3, Fig. 1 ).

There appeared to be no difference in the distribution of anti-A and anti-B activity in group O serum, though in two group O donors ( J. F. and E. M. ) only one type of agglutinin was found in the regions of low anionic binding capacity ( Figs. 1 and 2 ).

when the insoluble fraction was suspended in a volume of saline equal to that of the original serum sample, no complete antibody activity could be detected.

the serum glutamic oxaloacetic transaminase was 10 units per ml. per min..

The non-identity of serum and red blood cell arylesterase was also established.

In other words, a meter glucose reading of 39 mg / dL could be properly obtained from a person whose laboratory serum glucose was 53 mg / dL ; even wider variations can occur with " real world " home use.

On February 3, 1925, Gunnar Kaasen was first in the 1925 serum run to Nome to deliver diphtheria serum from Nenana, over 600 miles to Nome.

Though Balto is the more famous of the two, and was the dog to deliver the serum to Nome after running the final 53-mile leg, it was Togo who made the longest run of the relay, guiding his musher Leonhard Seppala on a 91-mile journey that included crossing the deadly Norton Sound to Golovin.

In 1963 the US Supreme Court ruled that confessions produced as a result of ingestion of truth serum was " unconstitutionally coerced ", and therefore inadmissible.

The first Nobel Prize in Physiology or Medicine was awarded in 1901 to the German physiologist Emil Adolf von Behring, for his work on serum therapy and the development of a vaccine against diphtheria.

He won the first Nobel Prize in Physiology or Medicine in 1901 for developing a serum therapy against diphtheria ( this was helped by Kitasato Shibasaburo and worked on with Emile Roux ) and tetanus.

Behring demonstrated that the injection of toxins was able to be transmitted to another animal by injections of a treated animal's blood serum and used as a means of effecting a cure.

Landsteiner described the regular patterns in which reactions occurred when serum was mixed with red blood cells, thus identifying compatible and conflicting combinations between these blood groups.

In 1925, Nome was the destination of the famous " Great Race of Mercy ", where dog sleds played a large part in transporting diphtheria serum through harsh conditions.

Nenana was the starting point for the 1925 serum run to Nome, where diphtheria antitoxin was transported by rail from Anchorage, and continued by dog sled to Nome.

The anti-human sera used were prepared by injecting whole human serum into rabbits.

Blood glucose levels discussed in this article are venous plasma or serum levels measured by standard, automated glucose oxidase methods used in medical laboratories.

Inoculation is the placement of something that will grow or reproduce, and is most commonly used in respect of the introduction of a serum, vaccine, or antigenic substance into the body of a human or animal, especially to produce or boost immunity to a specific disease.

* Trichloroacetic acid, chemical used to precipitate protein in serum

A truth drug or truth serum is a psychoactive medication used to obtain information from subjects who are unable or unwilling to provide it otherwise.

Antibody presence in blood serum is often used to determine whether a person has been exposed to a given virus in the past, with tests such as ELISA.

The drug is also used in people with gout or hyperuricemia ( high serum uric acid levels ).

A fasting serum insulin level of greater than the upper limit of normal for the assay used ( approximately 60 pmol / L ) is considered evidence of insulin resistance.

Repeated attacks can be prevented by different drugs used to reduce the serum uric acid levels.

Measuring serum creatinine is a simple test and it is the most commonly used indicator of renal function.

A major disadvantage of the indirect ELISA is the method of antigen immobilization is not specific ; when serum is used as the source of test antigen, all proteins in the sample may stick to the microtiter plate well, so small concentrations of analyte in serum must compete with other serum proteins when binding to the well surface.

Two specific antibodies are used, one conjugated with enzyme and the other present in serum ( if serum is positive for the antibody ).

Under the influence of a truth serum, Catharine tells the gruesome story of Sebastian's death by cannibalism at the hands of locals whose sexual favors he sought, using Catharine as a device to attract the young men ( as he had earlier used his mother ).

Steroids are generally ineffective, though are often used to combat serum sickness caused by ATG use.

In autologous immune enhancement therapy ( AIET ) the in vitro expanded NK cells are used along with T cells taken from the patients own peripheral blood and cultured without using feeder layers or animal serum.

The majority of biochemistry tests are performed on serum and so either a plain tube or a clotting accelerator is used.

Serological tests ( detection of antibodies in the serum of the patients ) are available and widely used.

An improvement arrived with the frog test, introduced by Lancelot Hogben, which still was used in the 1950s and allowed the frog to remain alive and be used repeatedly: a female frog was injected with serum or urine of the patient ; if the frog produced eggs within the next 24 hours, the test was positive.

Bites can be effectively treated with CroFab antivenom, as this serum is derived using venom components from four species of American pitvipers ( the eastern and western diamondback rattlesnakes, the Mojave rattlesnake and the cottonmouth ).

An animal study by Smith and Schou suggests that the kidneys clear lithium orotate significantly less effectively than lithium carbonate, and that this accounts for the higher serum levels of lithum when lithium orotate is taken compared to the equivalent amount of lithium carbonate.

The CuAAC click reaction also effectively couples polystyrene and bovine serum albumin ( BSA ).