When 1 ml of conjugate was passed through a column ( Af ), the first and second milliliter fractions collected were the most specific and gave no nonspecific staining in some experiments, and very little in others.

After these treatments the conjugate did not stain healthy or crown gall sweet clover tissues or stained them a very faint green which was easily distinguishable from the bright yellow-green specific staining.

In all cases a disturbing amount of nonspecific staining was still present although it was still distinguishable from specific fluorescence.

These conjugates Af had much less nonspecific staining than the previous conjugate ( with 50 mg FITC per gram of globulin ) while the specific staining was similar in both cases.

Results of specific staining by the direct and the indirect methods were similar and showed the localization of WTV antigen in certain tissues of tumors.

In our work the best procedure for removing substances causing nonspecific staining in order to obtain specific conjugates was to pass the conjugates through a DEAE-cellulose column and in some cases to absorb the first and second milliliter fractions with sweet clover tissue powder.

Further information can be gained by staining slices of brain tissue with a variety of chemicals that bring out areas where specific types of molecules are present in high concentrations.

Since the proteins remain in the native state they may be visualised not only by general protein staining reagents but also by specific enzyme-linked staining.

The first step in immunoperoxidase staining is the binding of the specific ( primary ) antibody to the cell or tissue sample.

Macrophages can be identified by specific expression of a number of proteins including CD14, CD40, CD11b, F4 / 80 ( mice )/ EMR1 ( human ), lysozyme M, MAC-1 / MAC-3 and CD68 by flow cytometry or immunohistochemical staining.

This can be used to specifically determine the percentage of lymphocytes that contain a particular combination of specific cell surface proteins, such as immunoglobulins or cluster of differentiation ( CD ) markers or that produce particular proteins ( for example, cytokines using intracellular cytokine staining ( ICCS )).

Quinacrine banding ( Q-banding ) was the first staining method used to produce specific banding patterns.

Diagnosis is usually performed by needle aspiration biopsy or excisional biopsy of the mass and the histological demonstration of stainable acid-fast bacteria in the case of infection by M. tuberculosis ( Ziehl-Neelsen stain ), or the culture of NTM using specific growth and staining techniques.

For pathological and biological research, the specimen usually undergoes a complex histological preparation that may involve cutting it into very thin sections with a microtome, fixing it to prevent decay, removing any water contained in it, staining specific parts of it, and impregnating or infiltrating it with some transparent solid substance.

The usual purpose is to reveal cytological details that might otherwise not be apparent ; however, staining can also reveal where certain chemicals or specific chemical reactions are taking place within cells or tissues.

One of the main difficulties with IHC staining is overcoming specific or non-specific background.

For identification in tissue, Mucicarmine stain provides specific staining of polysaccharide cell wall in Cryptococcus neoformans.

There are related stains known as the buffered Wright stain, the Wright-Giemsa stain, and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include Eosin Y, Azure B, and Methylene Blue ( some commercial preparations combine solutions to simplify staining ).

The most common test used to diagnose Paget's disease is the biopsy, removal of a tissue sample from the affected area which is then examined under the microscope by a pathologist, who distinguishes Paget cells from other cell types by staining tissues to identify specific cells ( immunohistochemistry ).

After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining ( for example, silver staining of proteins ), autoradiographic visualization of radioactive labelled molecules ( performed before the blot ), or specific labelling of some proteins or nucleic acids.

This monoclonal antibody shows specific immuno-cytochemical staining of nucleoli.

Let us not confuse the issue by labeling the objective or the method `` psychoanalytic '', for this is a well established term of art for the specific ideas and procedures initiated by Sigmund Freud and his followers for the study and treatment of disordered personalities.

The symposium provides an opportunity to confront the self with specific statements which were made at particular times by identifiable communicators who were addressing definite audiences -- and throughout several hundred pages everyone is talking about the same key symbol of identification.

This organization will differ from existing assistance programs in that its members will supplement technical advisers by offering the specific skills needed by developing nations if they are to put technical advice to work.

The Government of India further agrees in cooperation with the Government of the United States, to coordinate the use of grant and loan funds provided for in paragraphs 1 ( B ) and 1 ( C ) of Article 2, with such direct dollar assistance as may be made available by the Government of the United States of America, so that both sources of financing may be channeled to specific and clearly identifiable economic development programs and projects.

Mr. Claude is a specialist in torso development and he has long favored the now-famous Weider Push-Pull Super-Set technique in which one exercise of the Super-Set is a pressing or `` pushing '' movement which accents one sector of a muscle group in a specific way, followed by a `` pulling '' exercise which works the opposing sector of the same muscle group.

The reflection of radiation originating from the anode holder and reflected back to it by the surrounding metal surfaces should also be small because of the peculiar characteristic of the metal surfaces and of the specific geometry.

The behavior of a biological aerosol, on a much smaller scale, is illustrated by a specific field trial conducted with a non-pathogenic organism.

A solid immunity is the one effective circumstance whereby attack by a specific agent can be neutralized.

After passing Af through DEAE-cellulose, the titer of antibodies to WTV in the specific fraction was 1: 4 of the titer before such passage ( precipitin ring tests by R. F. Whitcomb ) ; ;

To establish an emotionally meaningful relationship the worker must demonstrate actual or potential helpfulness immediately, preferably within the first interview, by meeting the client's specific needs.

In this view, supported by only three members of the Court, a power denied by the specific provisions of Article 3, was granted by the generality of Article 1.

Though it is not easy to apply the evidence of the Iliad to any specific era, this marvelous product of the epic tradition had certainly taken definitive shape by 750.

In the specific case of time diffusion, we must emphasize the significance of the earlier development of mistrust when it is combined with the inevitable time crisis experienced by most ( if not all ) adolescents in our society, and with the failure of the adolescent period to provide opportunities for developing trust.

anti-discriminatory statutes in housing have now been adopted by thirteen states and, while specific provisions have varied, the tendency is clearly toward expanding coverage.

Virgilia Peterson, a critic by trade, has turned her critical eye pitilessly and honestly on herself in an autobiography more of the mind and heart than of specific events.

The style is characterized by specific rhythms and of Qacidate ( Popular poems ) in Arabic dialect that are long poems from the Algerian heritage.

The WD-2 robot can change its facial features by activating specific facial points on a mask, with each point possessing three degrees of freedom.

Some belief systems, such as those in the Abrahamic tradition, hold that the dead go to a specific plane of existence after death, as determined by a god, gods, or other divine judgment, based on their actions or beliefs during life.

The Spectrogram produced by such an instrument is a graphical display of the time varying pressure level and frequency profiles which give a specific acoustic signal its defining character.

The activities of the Planning Division are defined in considerable detail in the enabling act of the Development Council, which assigns to the agency both broad responsibilities and specific duties in the field of planning.

Even beginning students in linguistics are made familiar with an appreciable variety of consonant systems, both in their general outlines and in many specific details.

He is talking about double effects, of which the specific action causes directly the one and indirectly the other, but causes both ; ;

Initially a study of systems of polynomial equations in several variables, the subject of algebraic geometry starts where equation solving leaves off, and it becomes even more important to understand the intrinsic properties of the totality of solutions of a system of equations, than to find a specific solution ; this leads into some of the deepest areas in all of mathematics, both conceptually and in terms of technique.

Whilst the magnification of both the field ion and atom probe microscopes is extremely high, the exact magnification is dependant upon conditions specific to the examined specimen, so unlike for conventional electron microscopes, there is often little direct control on magnification, and furthermore, obtained images may have strongly variable magnifications due to fluctuations in the shape of the electric field at the surface.

These test cases must each be accompanied by test case input data or a formal description of the operational activities ( or both ) to be performed — intended to thoroughly exercise the specific case — and a formal description of the expected results.

to induce specific growth patterns in that one can easily prepare a solution containing the desired amount of hormone, add it to the known volume of GM, and autoclave to both sterilize and evaporate off any solvent that may have been used to dissolve the often-polar hormones.

The conflict in the border region of Darfur has become an increasingly bi-national affair as increasing numbers of Sudanese flee to refugee camps in Chad, and Sudanese government troops and militias cross the borders to strike at both these camps and specific ethnic groups.

In the military sphere, authorities can prosecute both regular crimes and specific acts ( such as mutiny or desertion ) under martial-law codes that either supplant or extend civil codes in times of ( for example ) war.

Cyclists form associations, both for specific interests ( trails development, road maintenance, urban design, racing clubs, touring clubs, and so on.

Despite this papal condemnation, both Catholicism and Protestantism permit the use of ambiguous and equivocal statements in specific circumstances.

In standard Greek usage, the older word " ecclesia " ( ἐκκλησία, ekklesía, literally " assembly ", " congregation ", or the place where such a gathering occurs ) was retained to signify both a specific edifice of Christian worship ( a " church "), and the overall community of the faithful ( the " Church ").

Linnaeus based the specific epithet marinus on an illustration by Dutch zoologist Albertus Seba, who mistakenly believed the cane toad to inhabit both terrestrial and marine environments.

An orienteering map combines both general and thematic cartography, designed for a very specific user community.

The general agreement is that Standard Danish is based on a form of Copenhagen dialect, but the specific norm, as with most language norms, is difficult to pinpoint for both laypeople and scholars.

However, most Indian languages have specific words that mean music or in some way denote it, for example ' Sangeeth ' in Hindi and ' Sangeetham ' in Malayalam both mean music.

These databases can include both segments shared by multiple sites, and segments specific to one site and used only locally in that site.

Broadly it deals with protecting specific chunks of data, both physically ( i. e., from corruption, or destruction, or removal ; e. g., see Physical security ), or the interpretation of them, or parts of them to meaningful information ( e. g., by looking at the strings of bits that they comprise, concluding specific valid credit-card numbers ; e. g., see Data encryption ).

Discoveries of both men contributed to the concept of localization, which states that specific brain functions are all localized to a specific area of the brain.

This essay-letter composed by Paul was written to a specific audience at a specific time ; to understand it, the situations of both Paul and the recipients must be understood.