In attempting to improve specificity of staining, the fluorescein-labeled antisera used in both direct and indirect methods were treated in one of several ways: ( 1 ) They were passed through Dowex-2-chloride twice and treated with acetone insoluble powders ( Coons, 1958 ) prepared from mouse liver or from healthy sweet clover stems or crown gall tissue produced by Agrobacterium tumefaciens ( E. F. Smith & Townsend ) on sweet clover stems.

They were for several hundred years frontier fortresses belonging to the French crown and most of what you will see there today in their well preserved remains dates from a post-Cathar era.

They lived in an isolated patch of eastern France that remained loyal to the French crown despite being surrounded by Burgundian lands.

They were second-class citizens and played no part in politics or law, and owed no military service to the crown, although in some cities they may have been the majority of the population.

They achieved the development of a permanent administrative system based on a class of public officials answerable to the crown.

They are however not among the crown group reptiles, and while the early groups may have been reptile-like in biology, the later ones were decidedly not reptile-like, nor were they true mammals.

They are the oldest surviving crown jewels in the United Kingdom.

They were ruled by officials appointed by the crown, known as wardens, prefects, or margraves.

They enter, and are welcomed by a woman in a gold crown.

They were moved inside the Museum of Memphis, and depicted the pharaoh standing in the attitude of the march, wearing the white crown of Upper Egypt, Hedjet.

They had Serrano ahead on points after 10 rounds, so the fight, and the crown, were given back to Serrano by a technical decision.

They saw each other but seldom, for Ferrante was one of the most active and brilliant captains of Charles V ; but Vittoria's influence was sufficient to keep him from joining the projected league against the emperor after the battle of Pavia ( 1525 ), and to make him refuse the crown of Naples offered to him as the price of his treason.

They place a purple robe on him, put a crown of thorns on his head, and according to Matthew, put a rod in his hand.

They are traditionally knitted from wool, and can be rolled up into a hat to cover just the crown of the head, or folded right down as a collar around the neck.

They arrive at a clearing where they witness a fight between a lion and a unicorn over a golden crown.

They shall be on the head of Joseph, And on the crown of the head of him who was separate from his brothers .” -

They also were used as dedicatory offerings to the deities, and in the dramatic contests at the Dionysia the victorious choregus ( a wealthy citizen who bore the expense of equipping and training the chorus ) received a crown and a tripod.

They are mainly brown, with a darker crown and back.

They crown two hills south-east of Göttingen, over Bremke.

They have a " crown " of solid tentacles whose cilia generate water currents that draw food particles towards the mouth, and both the mouth and anus lie inside the " crown ".

They offered the crown to Peter ( Petar ), whose relation to his predecessors is not mentioned by Orbin.

They are mainly grey and white plumaged, with a red beak ( as long as the head, straight, with pronounced gonys ) and feet, white forehead, a black nape and crown ( streaked white ), and white cheeks.

On the opposite side of the archway to that inscription is the shorter dedication: " They shall receive a crown of glory that fadeth not away ".

They tried to outface him, but Joseph Simmons was as wide as two average men, and it would have taken braver men than these were to outface him.

They gathered roots, bulbs, odd ferns, leaves, and bits of resin from the rare Santa Lucia fir, which exists only on a forty-five mile strip on the westerly side of these mountains.

`` They require, for existence, a brave spirit and a high heart, and where do you find these??

They showed no marked dependence on the flow rate within the accuracy of these measurements.

They had come from airports in the Balkans, these hundred-odd Junkers 88's.

They learn to like these so well that it isn't surprising to hear that one boy tried the oats he was feeding his horse at chore time.

They tell you horses go crazy at the sight or smell of a bear or a lion, but these didn't.

`` They cannot do otherwise than live in dread of each other since these weapons imply the possibility of such grisly surprise attack.

They did not need letters for the guttural sounds represented by aleph, he, heth or ayin, so these symbols were assigned vocalic values.

They generally have other terms specific to U. S. nationals, such as German US-Amerikaner, French étatsunien, Japanese 米国人 beikokujin, Arabic أمريكاني amriikaanii ( as opposed to the more-common أمريكي amriikii ), and Italian statunitense, but these may be less common than the term American.

They receive the vows of the nuns of the abbey ; they may admit candidates to their order's novitiate ; they may send them to study ; and they may send them to do pastoral and / or missionary work and / or assist — to the extent allowed by canon and civil law — in the administration and ministry of a parish or diocese ( these activities could be inside or outside the community's territory ).

They reproduce slowly either by bulb division or seeds and have gradually naturalized from plantings in urban and suburban areas throughout the lower elevations and coastal areas in much of the West Coast of the USA since these environments mimic their native South African habitat.

They have fraudulently added these uncontrolled, reckless, atheist descriptions in our texts.

They may be less effective than benzodiazepines in patients who have been previously treated with benzodiazepines as they do not provide the sedation that these patients may expect or equate with anxiety relief.

They rented the apartment of an American man who was away for the summer, and Nin came across a number of French paperbacks: " One by one, I read these books, which were completely new to me.

Plate anemometers have been used to trigger high wind alarms on bridges. They are used on these high places because they are in a plate shape ; has a good measurement status on higher altitudes.

They published the plans for all of these, with detailed design calculations and blueprints.

They called these parkas “ Kameikas ” for raingear in the English language K ( Aleut Corp.

" They also apply these judgments to other things that they encounter, and will usually deem something " cool " if it is associated with violence, sex or the macabre.

They send these signals by means of an axon, which is a thin protoplasmic fiber that extends from the cell body and projects, usually with numerous branches, to other areas, sometimes nearby, sometimes in distant parts of the brain or body.

They played 19 games of Australian rules football, against prominent clubs in Victoria and South Australia, winning six and drawing one of these ( see Australian rules football in England ).

They admit that these are not genuine names.

* They hold that the practice within Independent groups of ordaining women demonstrates an understanding of Priesthood that they vindicate is totally unacceptable to the Catholic and Orthodox churches as they believe that the Universal Church does not possess such authority ; thus, they uphold that any ceremonies performed by these women should be considered being sacramentally invalid.

“ They may produce global epidemics and impair the health of future generations .” He then stated that, in recognition of these dangers, the United States had decided to destroy its entire stockpile of biological agents and confine its future biological research program to defensive measures, such as vaccines and field detectors.

They overlap in range with 3 other crane species but interactions with these species and other " large wader " type birds are not known.