After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.

Moreover, all three representations must be squeezed comfortably into little more than the length Brumidi allowed for each one of his.

Five per cent of the voters in each county must sign petitions requesting that the Republicans be allowed to place names of candidates on the general election ballot, or 2

At such hearings each party is allowed a brief presentation at which the appellate judges ask questions based on their review of the record below and the submitted briefs.

Gradually, the detective interrogates each suspect, examines the scene of the crime and makes a note of each clue, so readers can analyse it and be allowed a fair chance of solving the mystery themselves.

Where Edwin Othello Excell sought to make the singing of " Amazing Grace " uniform throughout thousands of churches, records allowed artists to improvise with the words and music specific to each audience.

The size of each element, and the minimum and maximum values allowed for each index may also be included in the dope vector.

For each market area, the United States Federal Communications Commission ( FCC ) allowed two licensee ( networks ) known as " A " and " B " carriers.

The set of agreements used by a partnership about the meaning of each call is referred to as a bidding system, full details of which must be made available to the opponents ; ' secret ' systems are not allowed.

Bosonic particles, which include the photon as well as atoms such as helium-4, are allowed to share quantum states with each other.

The amended terms allowed former members of multiple societies which merge into one to maintain multiple entitlements to FSCS protection until 30 September 2009 ( later extended to 30 December 2010 ), so ( for example ) a member with £ 50, 000 in each of Nationwide, Cheshire and Derbyshire at the time of the respective mergers would retain £ 150, 000 of FSCS protection for their funds in the merged Nationwide.

In each case, the new body was allowed to use the regalia of the old corporation, and appoint ceremonial office holders such as sword and mace bearers as provided in their original charters.

Not all meanings of each word are allowed.

Once in every game, each king is allowed to make a special move, known as castling.

Dairy cows can only produce milk after having calved, and so every dairy cow is allowed to produce one calf each year throughout her productive life.

With a screen of tiny dots on each printing plate, the dots allowed an image to be printed in a halftone that appears to the eye in different gradations.

The scheme functions slightly differently in each area, and is managed by each branch, but the overall rule is that each participating pub is allowed to purchase beer from whatever brewery they wish, but if it the beer is to be promoted as a ' LocAle ' it must come from a brewery within a predetermined number of miles ( which is set by each CAMRA branch, but generally is around 20 or 25 miles, although, the North London branch has set it at 30 miles ) from brewery to pub door, even if it comes from a distribution centre further away ; in addition, each participating pub must keep at least one LocAle for sale at all times.

The conflict was only resolved by an interim agreement which allowed each emperor to support their preferred clergy within their own spheres of influence.

In this game each side fielded eleven men, participants were allowed to pick up the inflated egg-shaped ball and run with it and the ball carrier was stopped by knocking him down or " tackling " him.

Very old red wines often require several hours of aeration, and any red wine, brought from the cellar within half an hour of mealtime, should be uncorked and allowed some air.

Also, the sale of liquor is not allowed at all in nearly half of the state's counties.

By cutting transportation costs in half or more it became a large profit center for Albany, New York and New York City as it allowed the cheap transportation of many of the agricultural products grown in the mid west of the United States to the rest of the world.

In platform events, competitors are allowed to perform their dives on either the five, seven and a half ( generally just called seven ) or ten meter towers.

The headmaster undertook to help Blair to win the scholarship, and made a private financial arrangement that allowed Blair's parents to pay only half the normal fees.

A half page debuted the following Sunday ( March 29 ), with the strips for March 14 and 21, 1982, having a unique nine-panel format, but UFS curtailed further use of it ( but it allowed Davis to use the format for his U. S. Acres strip ).

During the late 13th century and first half of the 14th century, Chinese under the Mongol-controlled Yuan Dynasty were not allowed to enter higher posts of government ( reserved for Mongols or other ethnic groups from Central Asia ), and the Imperial examination was ceased for the time being.

On 15 June they met the two English ships, which Schrobop allowed to remain, on condition they gave half their catch to the Dutch.

This mutation has allowed almost half of the world ’ s population to metabolize lactose without symptoms.

Shortly after his accession at the conclusion of the papal election of December 1187, Clement succeeded in allaying the conflict which had existed for half a century between the Popes and the citizens of Rome, with an agreement by which the citizens were allowed to elect their magistrates, while the nomination of the governor of the city remained in the hands of the Pope.

Each monk is allowed a pound of bread and a hemina ( probably about half a pint ) of wine.

As a result of this play, NFL rules were changed so that, in the last two minutes of a half or game, the only offensive player allowed to advance a fumble is the player who originally fumbled.

Adal's use of firearms, still only rarely used in Ethiopia, allowed the conquest of well over half of Ethiopia, reaching as far north as Tigray.

This allowed complex disk-based software copy protection, by storing data on half tracks ( 0, 1, 2. 5, 3. 5, 5, 6 ...), quarter tracks ( 0, 1, 2. 25, 3. 75, 5, 6 ...), and any combination thereof.

There had been discussion among the Western Allies to place the southern Polish-German boundary on the Glatzer Neisse, which meant post-war Germany would have been allowed to retain approximately half of Silesia, including Breslau.

This separation made the 1S look like two " half Crays " separated by a few feet, which allowed the I / O system to be expanded as needed.

After graduating from high school in 1995, Shehhi enlisted in the Emirati military and received a half a year of basic training before he was admitted into a military scholarship program that allowed him to continue his education in Germany.

" This newly-recovered final line upset Samuel Noah Kramer's former interpretation, as he allowed: " my conclusion that Dumuzi dies and " stays dead " forever ( cf e. g. Mythologies of the Ancient World p. 10 ) was quite erroneous: Dumuzi according to the Sumerian mythographers rises from the dead annually and, after staying on earth for half the year, descends to the Nether World for the other half ".

His tactical and strategic insight allowed him to score huge gains against the Republic ( the New Essential Chrononlogy records that he added over a quarter of the galaxy to the Imperial's existing territory, allowing him control over half the galaxy ).

Unable to contain the fleeing of dollars, Echeverría allowed the peso to float for the first time on August 31, 1976, then again later and the peso lost half of its value.

If, however, the infant was allowed to survive half an hour only, it was spared ; but to have the right of keeping it the mother must find a male Areoi willing to adopt it.

A fumbled ball may be recovered and advanced by either team ( except, in American football, after the two-minute warning in either half or 4th down, when the fumbling player is the only offensive player allowed to advance the ball, otherwise the ball is ruled dead at the spot of recovery if the ball bounces backwards or spotted at the point of the fumble if the ball travels forward ).

An improvised landing sequence which started the retrorocket just 42 km above the moon ( about half the usual height ) allowed vernior engines to bring the craft down in 106 seconds from a height of only 1340 m ( about 10 % of the usual ).