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Page "Complementary DNA" ¶ 11
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# and synthesize
# The choline produced by the action of acetylcholinesterase is recycled — it is transported, through reuptake, back into the presynaptic terminal, where it is used to synthesize new acetylcholine molecules.
# to integrate ( or synthesize ) important facts, concepts, principles, and theories in IT management when developing solutions to IT management multifaceted problems in complex situations.
# A primer that matches the beginning of the DNA to sequence is used to synthesize a short DNA strand adjacent to the unknown sequence, starting with the primer ( see PCR ).
# Small molecules which enhance transdermal penetration: Research in this field is moving at a fast pace in order to synthesize small organic molecules which if injected in conjunction with siRNAs can help them penetrate into the target cells.

# and additional
# Telstar Sportsman ( 1978 ): Similar to Telstar Regent, but with an additional light gun and different setting switches.
# A structured literary creation by the evangelist which draws upon additional sources ;
# ICQ for Mac ( Beta )— Released in February 2010, a version of the client that was developed using the Adobe AIR platform, making it usable on additional operating systems which support the AIR runtime, such as Linux.
* The ANSI X9. 31 standard prescribes, IEEE 1363 describes, and PKCS # 1 allows, that p and q match additional requirements: be strong primes, and be different enough that Fermat factorization fails.
# Academic grade point average in all completed " a-g " courses, including additional points for completed University-certified honors courses.
# In a protocol applicable to a data unit, such as a block or frame, an additional item of information or a constraint encoded within the protocol to provide the required control.
# Any call for which an additional charge, i. e., toll charge, is not billed to the calling or called party, or ( depending on the country ) for which this charge is reduced because it is a short-distance call ( e. g. within a town or local metropolitan area ).
# Cinch the half hitches into a clove hitch, an additional clove hitch may be added if desired.
In April 2012, additional episode-only tags relating to plot points in an episode ( for instance, the # saturdaynightGLEEver tag for a mid-April 2012 episode of Glee with that episode title ) began to also be promoted in this space to both add additional trending topics and spread out more conversations on Twitter.
# Temple offering ( קרבן ): On the seven days of Sukkot, the additional ( מוסף ) offerings included 2 rams and 14 lambs each day, along with a series of 70 bulls in decreasing numbers over the week.
# " The additional security which its adoption will afford to the preservation of that species of government, to liberty and to prosperity "— covered in No. 85.
# so-called Browse connection to SWIFTNet ( also by SAG ) to use additional services, for example Target2
# Similarly, pleasure's opposite − pain − is universally avoided, which provides additional support for the idea that pleasure is universally considered good.
# Create additional candidates by creating several variants of each of the best candidates from the prior step
# Backlashes or other negative reactions to a transperson ’ s coming out are caused by transphobia and sexism, with additional homophobia and heterosexism in some cases.
# What additional effects does the new program include?
# Hull Shader: Performs operations on sets of patch control points, and generates additional data known as patch constants.
Two additional releases also went Top 40 in the US: " More Than You Know "(# 18 ) and " I Feel the Earth Move "(# 25 ), which was a remake of Carole King ’ s # 1 from her album Tapestry.
# Hold and Wait or Resource Holding: A process is currently holding at least one resource and requesting additional resources which are being held by other processes.
# Positive affect makes additional cognitive material available for processing, increasing the number of cognitive elements available for association ;
# Cannibal Ferox ( alternate title: Make Them Die Slowly ) – released with approximately 6 minutes of pre-cuts plus 6 seconds of additional cuts in 2000
# Revenge of the Bogey Man ( original title: Boogeyman II ) — released in re-edited form with additional footage in 2003
During the 55 years that he performed professionally, Burke released 38 studio albums on more than 17 record labels and had 35 songs that charted, including 26 songs that made the Billboard R & B charts, including " Got to Get You Off My Mind " that was # 1 in the summer of the 1965, and an additional 9 songs that were only listed on the Billboard Hot 100 chart, including 1964's seminal “ Everybody Needs Somebody to Love ”.

# and DNA
# The nuclear envelope becomes discontinuous and the DNA inside it is fragmented in a process referred to as karyorrhexis.
# DNA wraps around histone proteins forming nucleosomes ; the " beads on a string " structure ( euchromatin ).
# Higher-level DNA packaging of the 30 nm fibre into the metaphase chromosome ( during mitosis and meiosis ).
# Simple and concise definition: Chromatin is DNA plus the proteins ( and RNA ) that package DNA within the cell nucleus.
# A biochemists ’ operational definition: Chromatin is the DNA / protein / RNA complex extracted from eukaryotic lysed interphase nuclei.
# The DNA + histone
# A eukaryotic cell transcribes the DNA ( from genes ) into RNA ( pre-mRNA ).
# A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA ( Reverse transcriptase requires this double-stranded segment as a primer to start its operation.
# After digestion of the RNA, a single stranded DNA ( ssDNA ) is left and because single stranded nucleic acids are hydrophobic, it tends to loop around itself.
# From the hairpin loop, a DNA polymerase can then use it as a primer to transcribe a complementary sequence for the ss cDNA.
# REDIRECT DNA
# REDIRECT DNA microarray
# The DNA passes through a separate sugar transport protein ( ptsG ) in the inner membrane, and immediately circularises using the cos sites, 12-base G-C rich cohesive " sticky ends ".
# Host DNA gyrase puts negative supercoils in the circular chromosome, causing A-T rich regions to unwind and drive transcription.
# The host cell, containing a dormant phage genome, experiences DNA damage due to a high stress environment, and starts to undergo the SOS response.
# RecA ( a cellular protein ) detects DNA damage and becomes activated.
# Normally RecA * binds LexA ( a transcription repressor ), activating LexA auto-protease activity, which destroys LexA repressor allowing production of DNA repair proteins.
# Cleaved cI can no longer dimerise, and loses its affinity for DNA binding.
# The phage genome is still inserted in the host genome and needs excision for DNA replication to occur.
# C-terminal involved in downregulation of DNA binding of the central domain: residues 356-393.
# Complementary DNA then binds to the U5 ( non-coding region ) and R region ( a direct repeat found at both ends of the RNA molecule ) of the viral RNA
# The primer then ‘ jumps ’ to the 3 ’ end of the viral genome and the newly synthesised DNA strands hybridizes to the complementary R region on the RNA
# The first strand of complementary DNA ( cDNA ) is extended and the majority of viral RNA is degraded by RNAse H

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