However, its major drawback is that detailed structural knowledge of a protein is often unavailable, and even when it is available, it can be extremely difficult to predict the effects of various mutations.

One drawback to them is that the coldness of the iced drink may cause the tapioca balls to harden, making them difficult to suck up through a straw and chew.

This effectively triples the horizontal resolution of the image at normal viewing distances ; but the drawback is that the line thus drawn will show color fringes ( at some points it might look green, at other points it might look red or blue ).

As pointed out by Lange and Leiß, the drawback of these transformations is that they can lead to an undesirable bloat in grammar size.

This arrangement gives excellent efficiency, but can suffer from the drawback that there is a small mismatch in the cross-over region at the " joins " between the two halves of the signal, as one output device has to take over supplying power exactly as the other finishes.

" Lightfoot also notes that Eusebius cannot always be relied on: " A far more serious drawback to his value as a historian is the loose and uncritical spirit in which he sometimes deals with his materials.

However, the drawback is that it is memory intensive and it is therefore sometimes not used over Machin-like formulas.

The drawback of the four-card approach is that the Law of Total Tricks is more difficult to apply in cases where it is used.

The principal drawback of this approach is the high rate of false positive or equivocal levels due to the imprecision of the currently available meters: both physician and patient need an accurate understanding of what a meter can and cannot do to avoid frustrating and inconclusive results.

"</ ref > Although this efficiency limitation can be a drawback, an advantage of heat engines is that most forms of energy can be easily converted to heat by processes like exothermic reactions ( such as combustion ), absorption of light or energetic particles, friction, dissipation and resistance.

The drawback of this type of resistance mechanism is that the resistance is constant ; rowers using air or water resistance more accurately simulate actual rowing, where the resistance increases the harder the handle is pulled.

This drawback, however, does not produce significant loss of compression when the bitrate is already set at a high value for better quality ( as in the DVD mpeg2 format ).

The primary drawback is that high flows typically require larger sumps and bigger cooling units.

A secondary drawback is that a high flow system that relies on the flow rate to protect the lubricant from thermal stress is susceptible to catastrophic failure during sudden system shut downs.

The major drawback to this is the wide variation in quality of users ' audio cards.

A major drawback of current gesture interaction solutions is the lack of support for two necessary user interface design principles, feedback and visibility ( or affordance ).

A major drawback is that unlike OxyContin ( oxycodone continuous release ), methadone is not technologically engineered for sustained release of the drug so blood concentrations will fluctuate greatly between dosing.

In addition, a majority considered disemployment to be a moderate potential drawback to the increase they supported.

The primary drawback of using conventionally-tipped ballistic missiles is that they are virtually impossible for radar warning systems to distinguish from nuclear-tipped missiles.

People who observe drawback ( many survivors report an accompanying sucking sound ), can survive only if they immediately run for high ground or seek the upper floors of nearby buildings.

These estimators have a significant drawback in that they will drop a significant amount of sample data as only 1 / n of the available samples is being used.

The major drawback of these drugs is that they have properties that can lead to many side-effects ; for example, amitriptyline and doxepin both have antihistaminergic, anticholinergic, and antiadrenergic properties, which contribute to their side-effect profile, while mirtazapines side-effects are primarily antihistaminergic, and trazadones side-effects are primarily antiadrenergic.

The use of herbal anesthesia had a crucial drawback compared to modern practice — as lamented by Fallopius, " When soporifics are weak, they are useless, and when strong, they kill.

IIR filters, which are by nature recursive have the drawback that if not carefully designed they may enter limit cycles resulting in non-linear distortion.

The one great drawback, however, was that they had no money.

The main drawback to DQ is that novice characters and mighty heroes have nearly the same ability to absorb damage — i. e. they can both be killed fairly easily ( unlike D & D in which high-level characters can take remarkable amounts of damage without dying ).

One possible drawback to filters designed this way is that they contain many small ripples in the passband ( s ), since such a filter minimizes the peak error.

A drawback of the usual doubly special relativity models is that they are valid only at the energy scales where ordinary special relativity is supposed to break down, giving rise to a patchwork relativity.

A drawback of pure packet filters is that they are stateless ; they have no memory of previous packets which makes them vulnerable to spoofing attacks.

One drawback with spray drying is that the particles it produces are too fine to be used effectively by the consumer ; they must first be either steam-fused in towers similar to spray dryers or by belt agglomeration to produce particles of suitable size.

The drawback is that they require high-throughput, which is not feasible for all proteins.

Nowadays, with modern CPUs and better drivers, their most serious drawback is that they are operating system and machine dependent.

The drawback of self-extracting shell scripts ( any kind, not just shar ) is that they rely on a particular implementation of programs ; shell archives created with older versions of makeself, for example the original Unreal Tournament for Linux installer, fails to run on bash 3. x due to a change in how missing arguments to built-in command are handled.

All of the above methods, however, suffer from the same drawback: the person arrives at the location only to find out that they need to wait.

This, however, has the drawback of proliferating the rules, often to the point where they become difficult to manage.

Further, contact forms have the drawback that they require a website that supports server side scripts.

However, a potential drawback for content creators is that they can lose control over the presentation of their content when they syndicate it to other parties.

This removes the need for dedicated I / O instructions, although a drawback in such designs may be that special hardware must be used to insert wait states as peripherals are often slower than memory.

An additional drawback stems from the high rotor speed of this type of pump: very high grade bearings are required, which increase the cost.

Turing completeness in SQL is implemented through advanced standard features, illustrating one reason relatively powerful non-Turing-complete languages are rare: the more powerful the language is initially, the more complex are the tasks to which it is applied and the sooner its lack of completeness becomes perceived as a drawback, encouraging its extension until it is Turing complete.

Another drawback of the connectionless mode is that no optimizations are possible when sending several frames between the same two peers.

APUs providing electricity at 400 Hz are smaller and lighter than their 50 / 60 Hz counterparts, but are costlier ; the drawback being that such high frequency systems suffer from voltage drops.

The only drawback of using PCR to diagnose polycystic echinococcosis is that there aren ’ t many genetic sequences that can be used for PCR that are specific only E. oligarthus or E. vogeli.

However, a drawback to this architecture is that a large part of the system falls idle when programs or system tasks are executed that cannot be divided into units that can be processed in parallel.

The chief drawback is that what you are wolfing down is yourself.

The drawback is that Eldar vehicles are expensive to field in game.

Although pure silica is a perfectly viable glass and glass fiber, it must be worked with at very high temperatures, which is a drawback unless its specific chemical properties are needed.

A drawback of SECAM for production is that, unlike PAL or NTSC, certain post-production operations of encoded SECAM signals are not really possible without a significant drop in quality.

A drawback is that gallium compounds themselves are corrosive and so if the plutonium is recovered from dismantled weapons for conversion to plutonium dioxide for power reactors, there is the difficulty of removing the gallium.

The major drawback is that the stands are necessarily set back a good distance from the pitch, especially at the ends of the pitch.