For the albumin method, equal volumes of 30% bovine albumin, sample and 2% cells suspended in saline were allowed to stand at room temperature for 1 hr and then were centrifuged at 1000 rpm for 1 Aj.

The saline and albumin tests were performed as described for the ABO samples except that the mixture was incubated for 1 hr at 37-degrees-C before centrifugation.

Blood samples were allowed to clot at room temperature for 3 hr, centrifuged and the serum was removed.

The serum was measured volumetrically and subsequently dialyzed in the cold for at least 24 hr against three to four changes, approximately 750 ml each, of `` starting buffer ''.

Five milliamperes/cell were applied for 18 hr, after which the strips were stained with bromphenol blue and densitometry was carried out using a Spinco Analytrol.

At the end of the run, the strips in the third and sixth positions in each chamber were dried, stained for 1 hr, washed and dried, while the other strips were maintained in a horizontal position at 1-degree-C.

Fractions from the column which were to be subjected to analytical ultracentrifugation were concentrated by negative pressure dialysis and dialyzed for 16 hr in the cold against at least 500 volumes of phosphate-buffered saline, pH 7.2, ionic strength 0.154.

hr: 6. travnja

hr: 15. travnja

hr: 30. travnja

hr: 22. kolovoza

hr: 27. kolovoza

hr: 6. kolovoza

hr: 9. kolovoza

hr: 13. kolovoza

hr: 2. kolovoza

hr: 1. travnja

hr: 1. kolovoza

hr: 3. kolovoza

hr: 26. travnja

hr: 8. kolovoza

hr: 16. travnja

hr: 29. travnja

hr: 14. kolovoza

hr: 15. kolovoza

After a successful campaign Manuel I and Andronikos returned together to Constantinople ( 1168 ); but a year later, Andronikos refused to take the oath of allegiance to the future king Béla III of Hungary, whom Manuel desired to become his successor.

In 1168 Amalric and Manuel negotiated an alliance against Egypt, and William of Tyre was among the ambassadors sent to Constantinople to finalize the treaty.

In 1168 the chief Wendish fortress at Arkona in Rügen, containing the sanctuary of their god Svantevit, was conquered.

However, in 1168 the Fatimids under the leadership of Vizier Shawar set fire to Fustat to prevent Cairo's capture by the Crusaders.

Of all her influence on culture, Eleanor's time in Poitiers ( 1168 – 1173 ) was perhaps the most critical and yet very little is known about it.

" Franconia remained a Hohenstaufen power base until 1168, when the Bishop of Würzburg was formally ceded the ducal rights in Eastern Franconia.

Amalric cemented his alliance with Manuel by marrying Manuel's niece Maria Komnene in 1167, and an embassy led by William of Tyre was sent to Constantinople to negotiate a military expedition, but in 1168 Amalric pillaged Bilbeis without waiting for the naval support promised by Manuel.

During this time, he composed his acclaimed commentary on the Mishnah in the years 1166 – 1168.

Following this sojourn in Morocco, together with two sons, he sojourned in the Holy Land, before settling in Fostat, Egypt around 1168.

* In 1168, Maimonides published a comprehensive commentary on the Mishnah.

In 1168, the intercession of Pope Alexander III was necessary to secure a truce between them.

In 1168 he attacked the Normans at Builth, destroying its castle.

In 1168 during the Northern Crusades, Denmark mounted a crusade led by Bishop Absalon and King Valdemar the Great against the Wends of Rugia in order to convert them to Christianity.

* 1168: King Valdemar I of Denmark conquers Arkona on the Island of Rügen, the strongest pagan fortress and temple in northern Europe.

Year 1168 ( MCLXVIII ) was a leap year starting on Monday ( link will display the full calendar ) of the Julian calendar.

am: 1168 እ. ኤ. አ.

lt: 1168 m.

A bishop was appointed to Hebron in 1168 and the new cathedral church of St Abraham was built in the southern part of the Haram.

Henceforward Henry succeeded in keeping the countship of Anjou all his life ; for though he granted it in 1168 to his son Henry the Young King when the latter became old enough to govern it, he absolutely refused to allow him to enjoy his power.

* Robert de Beaumont, 2nd Earl of Leicester ( d. 1168 )

* William de Ferrers ( b. 1168 )

SDDS uses ATRAC1 with 8 channel encoding, and with a total encoding rate over all the channels of 1168 kbit / s.