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hr: Ahmed III.
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hr and .
For the albumin method, equal volumes of 30% bovine albumin, sample and 2% cells suspended in saline were allowed to stand at room temperature for 1 hr and then were centrifuged at 1000 rpm for 1 Aj.
The saline and albumin tests were performed as described for the ABO samples except that the mixture was incubated for 1 hr at 37-degrees-C before centrifugation.
Blood samples were allowed to clot at room temperature for 3 hr, centrifuged and the serum was removed.
The serum was measured volumetrically and subsequently dialyzed in the cold for at least 24 hr against three to four changes, approximately 750 ml each, of `` starting buffer ''.
Five milliamperes/cell were applied for 18 hr, after which the strips were stained with bromphenol blue and densitometry was carried out using a Spinco Analytrol.
At the end of the run, the strips in the third and sixth positions in each chamber were dried, stained for 1 hr, washed and dried, while the other strips were maintained in a horizontal position at 1-degree-C.
Fractions from the column which were to be subjected to analytical ultracentrifugation were concentrated by negative pressure dialysis and dialyzed for 16 hr in the cold against at least 500 volumes of phosphate-buffered saline, pH 7.2, ionic strength 0.154.
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