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Following the commercial success of this game, Nintendo released several follow-up titles in the Dr. Mario series.

Dr. Mario 64, released in 2001 for the Nintendo 64, features Wario and several Wario Land 3 characters, and offers numerous game modes, including a story-focused single player mode.

The game also supports simultaneous multiplayer for up to four players at once.

Dr. Mario 64 was subsequently released in Japan in the compilation game Nintendo Puzzle Collection on the Nintendo GameCube.

Dr. Mario Online Rx, released in 2008 on WiiWare, offers online multiplayer via Nintendo Wi-Fi Connection.

The most recent title, Dr. Mario Express, released in 2009 for the Nintendo DSi, does not support multiplayer gameplay.