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Paper electrophoresis was carried out on the concentrated samples in a Spinco model R cell using barbital buffer, pH 8.6, ionic strength 0.075, at room temperature on Whatman 3MM filter paper.
Five milliamperes/cell were applied for 18 hr, after which the strips were stained with bromphenol blue and densitometry was carried out using a Spinco Analytrol.
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