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Several samples of citrated plasma were fractionated in our laboratory by Method 6 of Cohn et al.
These fractions were tested for ABO agglutinin activity, using fractions from group AB plasma as a control.
As expected, most of the activity was found in Fraction Af, with slight activity seen in Fraction 4-1.
A sample of Fraction Af from group O plasma was dissolved in starting buffer, dialyzed against this buffer and subjected to chromatography using the gradient shown in Fig. 2.
Once again, both anti-A and anti-B activities were found in the insoluble material precipitated during dialysis.
Similarly, both types of antibodies were found in three regions of the chromatographic eluate, having extremely low, low, and high anionic binding capacity, respectively ( Fig. 3 ).
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