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Brown Corpus
In later experiments, Af and Af were prepared by conjugating 8 mg of FITC per gram of globulin.
These conjugates Af had much less nonspecific staining than the previous conjugate ( with 50 mg FITC per gram of globulin ) while the specific staining was similar in both cases.
Nonspecific staining could be satisfactorily eliminated by passing these conjugates through a DEAE-cellulose column as described for Af.
The best staining procedure with this purified Af consisted of staining with the conjugate for 30 minutes and washing in PBS for 15 minutes.
The specificity of staining with Af was established as follows: Af specifically stained tumor sections but not sections of healthy sweet clover stems or of crown gall tumor tissue from sweet clover.
Sections of tumors incited by WTV were not similarly stained with conjugated normal serum or conjugated antiserum to potato yellow-dwarf virus.
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