DNA may be visualized using ethidium bromide which, when intercalated into DNA, fluoresce under ultraviolet light, while protein may be visualised using silver stain or Coomassie Brilliant Blue dye.

The gel is dyed with Coomassie Brilliant Blue or silver to visualize the proteins.

Following electrophoresis, the gel may be stained ( most commonly with Coomassie Brilliant Blue R-250 or silver stain ), allowing visualization of the separated proteins, or processed further ( e. g. Western blot ).

Classical Coomassie Brilliant Blue staining can usually detect a 50 ng protein band, Silver staining increases the sensitivity typically 50 times.

The uniformity and overall effectiveness of transfer of protein from the gel to the membrane can be checked by staining the membrane with Coomassie Brilliant Blue or Ponceau S dyes.

* Brilliant Blue G, a type of Coomassie dye

To obtain a separation by size and not by net charge, as in IEF, an additional charge is transferred to the proteins by the use of Coomassie Brilliant Blue or lithium dodecyl sulfate.

These proteins can then be detected by a variety of means, but the most commonly used stains are silver and Coomassie Brilliant Blue staining.

BN-PAGE is a native PAGE technique, where the Coomassie Brilliant Blue dye provides the necessary charges to the protein complexes for the electrophoretic separation.

# REDIRECT Coomassie Brilliant Blue

The reagent Coomassie Brilliant Blue turns blue when it binds to arginine and aromatic amino acids present in proteins, thus increasing the absorbance of the sample.

The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250 in which under acidic conditions the red form of the dye is converted into its bluer form to bind to the protein being assayed.

* Coomassie Brilliant Blue 1

The zymogram is subsequently stained ( commonly with Amido Black or Coomassie Brilliant Blue ), and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme.

Classical Coomassie Brilliant Blue staining can usually detect a 50 ng protein band ; silver staining increases the sensitivity typically 50 times.

Immunoprecipitates may be seen in the wet agarose gel, but are stained with protein stains like Coomassie Brilliant Blue in the dried gel.

Two methods of analysis are possible: ( a ) protein electrophoresis, a method of analyzing the composition of fluids, also known as " agarose gel electrophoresis / Coomassie Blue staining ", and ( b ) the combination of isoelectric focusing / silver staining.

# Add 5. 0 mL of Coomassie Blue to each tube and mix by vortex, or inversion.

# Add 100 µL of each of the above to separate tubes ( use microcentrifuge tubes ) and add 1. 0 mL of Coomassie Blue to each tube.

In many cases this method has lower resolution than BN-PAGE, but CN-PAGE offers advantages whenever Coomassie dye would interfere with further analytical techniques, for example it has been described as a very efficient microscale separation technique for FRET analyses .< ref > Gavin P. D., Devenish R. J., Prescott M. FRET reveals changes in the F < sub > 1 </ sub >– stator stalk interaction during activity of F < sub > 1 </ sub > F < sub > 0 </ sub >- ATP synthase.

Coomassie blue ( also brilliant blue ) nonspecifically stains proteins a strong blue colour.

Each band can be detected using stains such as Coomassie blue dye or silver stain.

The disadvantage of Coomassie is that in binding to proteins it can act like a detergent causing complexes to dissociate.

During the formation of this complex, two types of bond interaction take place: the red form of Coomassie dye first donates its free electron to the ionizable groups on the protein, which causes a disruption of the protein's native state, consequently exposing its hydrophobic pockets.

The binding of the protein stabilizes the blue form of the Coomassie dye ; thus the amount of the complex present in solution is a measure for the protein concentration, and can be estimated by use of an absorbance reading.

When SDS concentrations are below critical micelle concentration ( known as CMC, 0. 00333 % W / V to 0. 0667 %) in a Coomassie dye solution, the detergent tends to bind strongly with the protein, inhibiting the protein binding sites for the dye reagent.

* Maia Mizuki, the protagonist of the anime Daphne in the Brilliant Blue

* FD & C Blue No. 1 – Brilliant Blue FCF, E133 ( blue shade )

Brilliant Blue ( BBG ) food coloring was cited in a recent study in which rats that had suffered a spinal injury were given an injection of the dye immediately after the injury, and were able to regain or retain motor control.

Those that enter and stain living cells are called supravital stains ( eg New Methylene Blue and Brilliant Cresyl Blue for reticulocyte staining ).

Died Pretty recorded their third album, Every Brilliant Eye, in Los Angeles with Jeff Eyrich ( The Gun Club, The Plimsouls ) producing, it was released on Blue Mosque in April 1990.

These are differentiated by having ' Cadmium Yellow ' coloured key for 3. 3V or ' Brilliant Blue ' colour for 5V operation.

* Daphne in the Brilliant Blue ( Shizuka Hayama, Woman )

* Daphne in the Brilliant Blue ( Tsutomu Hanaoka )

* Yu Park in Daphne in the Brilliant Blue

## Hemoglobin H Inclusions-alpha thalassemia, greenish-blue inclusion bodies appear in many erythrocytes after four drops of blood is incubated with 0. 5mL of Brilliant cresyl blue for 20 minutes at 37 ° C.

Vastly different from the pop rock sound of The Brilliant Green, Tommy february < sup > 6 </ sup >' s persona and sound are inspired by and derived from British and American 1980's synth pop, including a cover of Strawberry Switchblade's " Since Yesterday ".

Her campaign manager, Morton Brilliant, resigned shortly thereafter .< ref > Taylor ’ s son was charged with driving under the influence ( DUI ) after crashing his car on August 18, 2005 in Charleston, South Carolina, killing his passenger.

* David Sylvian: Brilliant Trees ( 1984, features Ryuichi Sakamoto on piano / synthesizers on 3 tracks )

3, Pieter Jan Leusink, Netherlands Bach Collegium, Sytse Buwalda, Brilliant Classics 1999

In April 2005, Infratil completed a major £ 3 million refurbishment of the terminal building, and also controversially rebranded the airport using the phrase " Pure Dead Brilliant ", taken straight from the Glasgow patter.

3, Pieter Jan Leusink, Netherlands Bach Collegium, Ruth Holton, Brilliant Classics 1999

#" My Brilliant Feat " ( Hay, Talbot ) – 3: 26

" A Brilliant Case for the Defense " ( February 3, 1986 ), Sports Illustrated.

Reaching 3 – 4 m ( 10 – 13 ft ) high, it is a larger plant than ' Braidwood Brilliant '.

* The Genius of Dino Ciani ; Debussy ( Préludes ), Schumann ( Novelettes ), von Weber ( Piano sonata's 2 and 3 ), Bartok ( Out of Doors, volume 2 ); Original recordings made by Deutsche Grammophon, Brilliant Classics, 2010, 3CD )