After dialysis the sample was centrifuged and the supernatant placed on a Af cm column of EEAE-cellulose equilibrated with starting buffer.

After introduction into the vacuum system, the sample is reduced to cryogenic temperatures ( typically 20-100 K ) and manipulated such that the needle's point is aimed towards an ion detector.

After the resin has been polymerised ( hardened ) the sample is thin sectioned ( ultrathin sections ) and stained – it is then ready for viewing.

After taking a small sample of the outer shell, the three hear a pair of voices, which informs them that the egg belongs to Mothra.

* Listen to music sample of Kokaji and Musume Dōjōji ( After clicking on this link, scroll down page )

After the atom is ionized it can be removed from the sample by applying an electric field.

After a filter is designed, it must be realized by developing a signal flow diagram that describes the filter in terms of operations on sample sequences.

After the last sub-channel, error correction, and synchronization, the cycle starts all over again with a new frame, starting with the second sample, byte or data block from sub-channel 1, etc.

After the agar cools and solidifies, the dish is ready to receive a microbe-laden sample in a process known as inoculation or " plating.

After the ghosts treat him to a sample of the agonies he would face, Robin reluctantly agrees.

After one hour, a second blood sample is drawn, and, after another hour, a third sample is taken.

After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.

After drilling for 7 minutes, the drill reached a stop at 35 centimeters depth and then withdrew its sample and lifted it in an arc to the top of the spacecraft, depositing the lunar material in a small spherical capsule mounted on the main spacecraft bus.

After collecting a small sample of lunar soil, the spacecraft ’ s ascent stage lifted off at 22: 58 UT on 22 February and quickly accelerated to 2. 7 kilometers per second velocity — sufficient to return to Earth.

After the sample has spun at high speed for sufficient time to produce the separation, the rotor is allowed to come to a smooth stop and the gradient is gently pumped out of each tube to isolate the separated components.

After each single measurement, the probe is rinsed with distilled water or deionized water to remove any traces of the solution being measured, blotted with a scientific wipe to absorb any remaining water which could dilute the sample and thus alter the reading, and then quickly immersed in another solution.

After the war he worked on sample survey design and analysis.

After being perturbed by interaction with the sample under test, the sample beam is recombined with the reference beam to create an interference pattern which can then be interpreted.

After some possible washing and / or pre-drying of this filtrate, the residue on the filter paper can be placed in the crucible and fired ( heated at very high temperature ) until all the volatiles and moisture are driven out of the sample residue in the crucible.

After releasing the sample return capsule on September 8, 2004, the spacecraft bus traveled back toward the Earth-Sun Lagrange Point ( L1 ).

After drawing a sample story and sketch for their editor and receiving approval, Ohkawa assigns the roles to each group member and then chooses the visual styles depending on factors such as the complexity of the story, the chosen art style, and its relationship to the group's other works.

After how many generations is such wealth ( mounting all the while through the manipulations of high finance ) purified of taint??

After allowing for group exposures, it is apparent that other factors must be considered if we are to comprehend fanaticism.

After all, Shelley is no `` orthodox '' or Hellenic Platonist, and even his `` romantic '' Platonism can be distinguished from that of his contemporaries.

After Quiney was elected bailiff in September, 1601, without Greville's approval, Greene wrote him that Coke had promised to be of counsel for Stratford and had advised `` that the office of bayly may be exercised as it is taken upon you, ( Sr. Edwardes his consent not beinge hadd to the swearinge of you ) ''.

After all, it goes back to the days in which sedition was not un-American, the days in which the Sons of St. Tammany conspired to overthrow the government by force and violence -- the British government, that is.

After all, when one has asked whatever became of old Joe and Charlie when one has inquired who it was Sue Brown married and where it is they now live when questions are asked and answered about families and children, and old professors when the game and its probable outcome has been exhausted that does it.

After the Griffin-Byrd political troup has completed the circuit in November in the name of a Pre-Legislative Forum, this is going to be the most politically oriented Legislature in history.

After a minute he went on, `` People must think the curse is on me, seeing you fresh as an apple and me old and gray ''.

After the recommendation of the Department is forwarded to the appeal board, that is the appropriate place for a registrant to lodge his denial.

After you have taken a breather, reverse the position of your legs so that the front thigh of the previous exercise is now to the rear, and the rear thigh now to the front, and perform the same movement in the same manner.

After notching it for the keelson, chines and battens, the half-inch plywood transom is secured to it with glue and the same type nails.

After the frames and transom are set up on the jig and temporarily braced, a piece of three-inch-wide mahogany ( only widths will be given since the 13/16-inch thickness is used throughout ) is butted between frames one and two below the line of the keelson.

After trimming off the excess on the frames and transom which was used to fasten them to the jig at a working height, the top of the side planking is installed.

After the decking is on, the cabin sides are installed.

After it has been seen, have the child start on a mat on hands and knees ( a thin, inexpensive mat is quite sufficient for anything that does not require falling ).

After a few tortuous moments of wondering who `` he '' is, the camera pans across the room to the plaster statue, and we realize that Neitzbohr is trying to redeem himself in the eyes of a mute piece of sculpture.

After it has reached terminal velocity, the time for the tape to travel a known distance is recorded.

After the male and female andrenas have mated, the cycle is repeated.

After all, social life in the group of the bees is by no means general, although it certainly is a striking feature.

After being located for some years in the Village at the Equinox Pharmacy under the supervision of Mrs. Harry Mercier, it is presently located in the Hill and Dale Shop, Manchester Center.

After 1895 the number increases, and in the next thirty years there is only one year for which there is no dated poem -- 1903, when Hardy was at work on The Dynasts.