Despite some claims that shotgun sequencing was in some ways less accurate than the clone-by-clone method chosen by the Human Genome Project, the technique became widely accepted by the scientific community and is still the de facto standard used today.

Despite the advantages of high spatial resolution and precise chemical sensitivity attributed to AES, there are several factors that can limit the applicability of this technique, especially when evaluating solid specimens.

Despite these sometimes substantial drawbacks, Auger electron spectroscopy is a widely used surface analysis technique that has been successfully applied to many diverse fields ranging from gas phase chemistry to nanostructure characterization.

Despite organizational changes and while additional techniques were added, the Transcendental Meditation technique itself remained relatively unchanged.

Despite his technique, he was unable to play without vibrato.

Despite the rhetorical distaste all serial authors used the cliffhanger and Wilkie Collins is famous for saying about the technique: " Make ' em cry, make ' em laugh, make ' em wait – exactly in that order.

Despite the popularity of modern-swing technique in Lindy Hop circles in the US and worldwide, many dancers in Lindy Hop communities prefer to stick to the old tradition.

Despite the decreasing use of the 3 pin " rat trap " (" rottefella " in Norwegian ) binding in lighter cross country, the characteristic " duckbill " toe it uses is still assumed in the design of heavier cable bindings, and 75 mm boots are still widely available, especially for telemark technique and more rugged touring.

Despite the success of I Love Lucy, most television comedies in the ' 50s and ' 60s used the single-camera technique, with a laugh track simulating the absent audience.

Despite the many stylised figurines, Olmec-period artisans and artist also portrayed humans naturalistically with " a most extraordinary realistic technique ".

Despite his committee releasing a report sceptical of hydraulic fracturing in the United Kingdom, Yeo has revised his personal opinion and supports the use of the technique in the UK.

Despite having only one leg, he was forced to stand in stress positions, " an acutely difficult technique for him " as the Americans took away his false leg, forcing him to balance awkwardly on one foot until losing his balance and ripping at the tendons in his arms.

Despite its relative technical simplicity, the injection ( grafting ) technique for breast augmentation is accompanied by post-procedure complications — fat necrosis, calcification, and sclerotic nodules — which directly influence the technical efficacy of the procedure, and of achieving a successful outcome.

Despite multiple problems, the building of the Huddersfield Narrow canal showed that the technique of quantity surveying had advanced greatly.

Despite not being at full strength due to his injury leading to chronic problems, and his sealing of a powerful technique that required the use of his left arm, Tezuka eventually became known as a National-level tennis player.

Despite that, using the technique of singular extensions it could also follow lines of forced moves that reach even further, which is how it once found a checkmate in 37 moves.

Despite suffering from Parkinsons disease, Muehl continued his art work, and in 2002 developed " electric painting films ", a new technique in which he paints digital photos from actions using a computer tablet and pen and edits the process into films.

The technique is described in Dr. Farwell's paper " Using Brain MERMER Testing to Detect Concealed Knowledge Despite Efforts to Conceal ", published in the Journal of Forensic Sciences in 2001 by Dr. Farwell and FBI Supervisory Special Agent Sharon Smith of the FBI ( Farwell & Smith 2001 ).

Despite its many limitations ( compared to later methods it is inaccurate ; it can only be used on organic matter ; it is reliant on a dataset to corroborate it ; and it only works with remains from the last 10, 000 years ), the technique brought about a revolution in archaeological understanding.

Despite the documentary nature of many of his assignments, Baldus was no purist when it came to technique.

Despite the ostensible impossibility of such a technique, there were investors that saw potential.

Despite all these problems, in situ ellipsometry becomes more and more important as process control technique for thin film deposition and modification tools.

Despite relying upon a single lichen this technique is praised for its simplicity and allows obtaining an image of the age of rock exposure while still in the field.

Despite this, the low efficiency of the technique has prompted some researchers, notably Ian Wilmut, creator of Dolly the cloned sheep, to abandon it.

Despite this limited franchise, Athenian democracy was certainly the first — and perhaps the best — example of a working direct democracy.

Despite, or perhaps because of, its small size Harwich is highly regarded in terms of architectural heritage, and the whole of the older part of the town, excluding Navyard Wharf, is a conservation area.

Despite being modest in number ( perhaps 7, 000 units total as of 1988 ), Lisp machines commercially pioneered many now-commonplace technologies – including effective garbage collection, laser printing, windowing systems, computer mice, high-resolution bit-mapped graphics, computer graphic rendering, and networking innovations like CHAOSNet.

Despite its urban and industrial character, San Francisco Bay and the Sacramento-San Joaquin Delta remain perhaps California's most important ecological habitats.

Despite the influx of advertisers and their cash, or perhaps because of them, the 1940s were not without bumps for the radio networks.

Despite several references listing different combinations of Picti, Scotti, Hiberni, Attecotti and Saxons together as later Roman Britain's archetypal enemies, it is worth noting that ' Scotti ' and ' Hiberni ' are never listed together, perhaps suggesting that they were then, as they were later, alternative names for the Irish or confederations of the Irish.

Despite the vast size of its coma, Halley's nucleus is relatively small: barely 15 kilometers long, 8 kilometers wide and perhaps 8 kilometers thick.

Despite the lack of hard evidence, it has been put forward by some authorities that black robes, perhaps slightly tainted with red to hide bloodstains, was indeed the sensible garment of choice for infiltration.

Despite ( or perhaps because of ) the manner in which Ford intended it, Rose adopted that nickname as a badge of honor.

Despite this background ( or, perhaps, because of it ), poverty and oppression are the main subjects of Murenu's poetry.

Despite this, or perhaps because of it, the spirit of this mystic comes alive through a " unique forcefulness ... vigor of thought and rugged terseness of style.

Despite ( or perhaps because of ) his pride and persistence, Sylvester is, with rare exceptions, placed squarely on the " loser " side of the Looney Tunes winner / loser hierarchy.

For all that, in June 1961 Bray still decided to move and despite his recent marriage “ lmost every day went round, often spending a good part of the day or a large part of the evening there .” “ Oddly enough, this side of his life was well known about in Paris … natural reserve and well-developed sense of decorum were allied to his fear of giving offence to Suzanne .” Anthony Cronin notes that strangely – or perhaps not so strangely – during this time he was often to be found talking “ fervently and seriously about suicide .” Despite his unwillingness to do much about it he was clearly suffering badly from guilt.

Despite the hard times they endured together, or perhaps because of them, Shaka loved his mother almost to the point of worship.

Despite the relative low number of Polynesian languages, and the relative abundance of data already available on many of them, the comparative method was often reduced to comparisons of vocabulary, its shared sporadic sound changes and, as Wilson did in 1985, comparison of pronominal systems which is perhaps the second most commonly described aspect of " minor " languages often available for comparison after the lexicostatistical lists.

Despite ( or perhaps because of ) their conflicting attitudes, the Professor and Thomas are billeted together by Satan.

Despite the intensive military operations, apparently no serious thought was given stripping Madison of his powers and duties temporarily, perhaps partly because his Vice President, Elbridge Gerry, also was in poor health and nearing age 70.

Despite the brutality Lamb got along well at Christ's Hospital, due in part, perhaps, to the fact that his home was not far distant thus enabling him, unlike many other boys, to return often to the safety of home.

Despite a worldwide course development slowdown, Nicklaus ’ s design firm has over 40 courses in development around the globe ... And he remains perhaps golf ’ s most respected spokesperson on a wide range of issues.

" Despite their insanity, or perhaps because of it, the Malkavians have the capacity to " see " the future.

Despite ( or perhaps because of ) the country's association with Joseph Stalin, Soviet rule in Georgia was particularly harsh during the 1950s and sought to restrict Georgian cultural expression.

Despite Perry's notoriety for doctoring baseballs ( i. e. throwing a spitball ), and perhaps even more so for making batters think he was throwing them on a regular basis – he even went so far as to title his 1974 autobiography Me and the Spitter – he was not ejected for the illegal practice until August 23, 1982, in his 21st season in the majors.

Despite this, or perhaps because of it, Louis XVIII forced Gazan into retirement in Grasse, where the aging general dabbled unsuccessfully in politics.

Despite this, perhaps partly again due to superstitution and partly because Emperor Ling viewed his behavior as being insufficiently solemn, Prince Bian was never created crown prince, and at times Emperor Ling seriously considered creating the younger Prince Xie crown prince.